{"id":112,"date":"2013-12-19T14:10:35","date_gmt":"2013-12-19T08:10:35","guid":{"rendered":"http:\/\/icg.nsc.ru\/isiah\/?p=112"},"modified":"2013-12-19T14:10:35","modified_gmt":"2013-12-19T08:10:35","slug":"112","status":"publish","type":"post","link":"http:\/\/sites.icgbio.ru\/isiah\/en\/2013\/12\/19\/112\/","title":{"rendered":"\u041e\u043b\u0438\u0433\u043e\u043d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434\u044b \u0434\u043b\u044f \u041f\u0426\u0420-\u0420\u0412"},"content":{"rendered":"

Oligo used for RT PCR<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Oligo name<\/b><\/p>\n<\/td>\n

\n

Sequence<\/b><\/p>\n<\/td>\n

\n

Oligo length<\/b><\/p>\n

b.<\/b><\/p>\n<\/td>\n

\n

Temp of <\/b>annealing<\/strong><\/p>\n<\/td>\n

\n

Product length<\/b> ,<\/b><\/p>\n

b.<\/b><\/p>\n<\/td>\n<\/tr>\n

\n

PpiaL<\/p>\n<\/td>\n

5\u2019-ACCGTGTTCTTCGACATCAC-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n

\n

130<\/p>\n<\/td>\n<\/tr>\n

\n

PpiaR<\/p>\n<\/td>\n

5\u2019-GAACCCTTATAGCCAAATCCT-3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n<\/tr>\n

\n

EgfrL<\/p>\n<\/td>\n

5\u2019-CGGAACTTACAGGA\u2193<\/sup>AATTCTG-3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n

\n

117<\/p>\n<\/td>\n<\/tr>\n

\n

EgfrR<\/p>\n<\/td>\n

5\u2019-CATGTTGCTCAGAAAGACATC-3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n<\/tr>\n

\n

ComtL<\/p>\n<\/td>\n

5\u2019-GACAA\u2193AGTCACCATCCTCAAT-3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n

\n

122.<\/p>\n<\/td>\n<\/tr>\n

\n

ComtR<\/p>\n<\/td>\n

5\u2019- GAAGTGTGTCTGGAAGGTAG -3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n<\/tr>\n

\n

EgfL<\/p>\n<\/td>\n

5\u2019- ACATCAAGCACG\u2193<\/sup>GCACGATT-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n

\n

112<\/p>\n<\/td>\n<\/tr>\n

\n

EgfR<\/p>\n<\/td>\n

5\u2019-ATTCTTCCCAGTGTGTTTGTC-3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n<\/tr>\n

\n

Scnn1aL<\/p>\n<\/td>\n

5\u2019-CTCCTTCT\u2193<\/sup>GTCACGATGGTC-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

62<\/p>\n<\/td>\n

\n

141<\/p>\n<\/td>\n<\/tr>\n

\n

Scnn1aR<\/p>\n<\/td>\n

5\u2019-GTAGCAGCATGAGAAGTGTG-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n<\/tr>\n

\n

bENaCL<\/p>\n<\/td>\n

5\u2019-CTGGGACTGAATTTG\u2193<\/sup>GTCTCA-3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

62<\/p>\n<\/td>\n

\n

127<\/p>\n<\/td>\n<\/tr>\n

\n

bENaCR<\/p>\n<\/td>\n

5\u2019-TCTCTAATGAAGGGGTATGTC-3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n<\/tr>\n

\n

Ephx2L<\/p>\n<\/td>\n

5\u2019-TATGTGACAGTGAAG\u2193<\/sup>CCAGG-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n

\n

192<\/p>\n<\/td>\n<\/tr>\n

\n

Ephx2R<\/p>\n<\/td>\n

5\u2019-GAAGATGAGTCTCCATAGCC-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n<\/tr>\n

\n

MlrL<\/p>\n<\/td>\n

5\u2019-CCAAGGTACTTCCAG\u2193<\/sup>GATTTA-3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n

\n

159<\/p>\n<\/td>\n<\/tr>\n

\n

MlrR<\/p>\n<\/td>\n

5\u2019- TTGGCTGTTCGTGTGTTTGTA -3\u2019<\/td>\n\n

21<\/p>\n<\/td>\n

\n

60<\/p>\n<\/td>\n<\/tr>\n

\n

\u00a0aENaCr1<\/p>\n<\/td>\n

5\u2019-AGTGTAATTGTTCTGCAAGGAC-3\u2019<\/td>\n\n

22<\/p>\n<\/td>\n

\n

62<\/p>\n<\/td>\n

\n

–<\/b><\/p>\n<\/td>\n<\/tr>\n

\n

aENaCwt<\/p>\n<\/td>\n

5\u2019-GCTCCTGGG\u2193<\/sup>GCTATTGCTAT-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

62<\/p>\n<\/td>\n

\n

238<\/p>\n<\/td>\n<\/tr>\n

\n

aENaCa<\/p>\n<\/td>\n

5\u2019-GCTCCTGGG\u2193<\/sup>GGCGCCTTCTC-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

70<\/p>\n<\/td>\n

\n

215<\/p>\n<\/td>\n<\/tr>\n

\n

aENaCb<\/p>\n<\/td>\n

5\u2019-GCTCCTGGG\u2193<\/sup>TGTGATCAACT-3\u2019<\/td>\n\n

20<\/p>\n<\/td>\n

\n

62<\/p>\n<\/td>\n

\n

159<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u2193<\/b> – division of exon<\/p>","protected":false},"excerpt":{"rendered":"

Oligo used for RT PCR<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/posts\/112"}],"collection":[{"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/comments?post=112"}],"version-history":[{"count":0,"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/posts\/112\/revisions"}],"wp:attachment":[{"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/media?parent=112"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/categories?post=112"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/sites.icgbio.ru\/isiah\/en\/wp-json\/wp\/v2\/tags?post=112"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}