{"id":56,"date":"2022-06-16T09:26:12","date_gmt":"2022-06-16T06:26:12","guid":{"rendered":"https:\/\/sites.icgbio.ru\/tutorial\/?page_id=56"},"modified":"2022-06-16T09:26:12","modified_gmt":"2022-06-16T06:26:12","slug":"sequence_translit","status":"publish","type":"page","link":"https:\/\/sites.icgbio.ru\/tutorial\/sequence_translit\/","title":{"rendered":"\u041d\u0430\u0431\u043e\u0440\u044b \u0434\u0430\u043d\u043d\u044b\u0445 \u0438\u00a0 \u043f\u043e\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u0442\u0435\u043b\u044c\u043d\u043e\u0441\u0442\u0438 \u0434\u043b\u044f \u043f\u0440\u0430\u043a\u0442\u0438\u0447\u0435\u0441\u043a\u0438\u0445 \u0440\u0430\u0431\u043e\u0442"},"content":{"rendered":"

\u0413\u043b\u0430\u0432\u0430 5.<\/h2>\n

\u0417\u0430\u0434\u0430\u0447\u0430 \u00ab\u0423\u0437\u043d\u0430\u0439, \u0447\u0442\u043e \u044d\u0442\u043e \u0437\u0430 \u0431\u0430\u043a\u0442\u0435\u0440\u0438\u044f, \u043f\u043e \u0414\u041d\u041a\u00bb<\/p>\n

GGGAGTAAAGTTAATACCTTTGCTC<\/p>\n

\u0413\u043b\u0430\u0432\u0430 7.<\/h2>\n

\u0417\u0430\u0434\u0430\u0447\u0430 \u00ab\u0427\u0442\u043e \u0432\u044b\u043a\u043b\u044e\u0447\u0430\u0435\u0442 \u0420\u041d\u041a\u00bb<\/p>\n

AAUUGUUCCACAAUGCCACGC<\/p>\n

\u0413\u043b\u0430\u0432\u0430 11.<\/h2>\n

\u0417\u0430\u0434\u0430\u0447\u0430 \u00ab\u041e \u0447\u0451\u043c \u043c\u043e\u0436\u0435\u0442 \u0441\u043a\u0430\u0437\u0430\u0442\u044c \u043d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434\u043d\u0430\u044f \u0437\u0430\u043c\u0435\u043d\u0430\u00bb<\/p>\n

\u041d\u043e\u0440\u043c\u0430<\/p>\n

ACCTTGGCTGTACCCCCTGGGGAAGAGCAGAGATATACGTGCCAGGTGGAGCACCCAGGCCTGGATCAGCCCCTCATTGTGATCTGGGGTATGTG<\/p>\n

\u041f\u0430\u0446\u0438\u0435\u043d\u0442<\/p>\n

ACCTTGGCTGTACCCCCTGGGGAAGAGCAGAGATATACGTACCAGGTGGAGCACCCAGGCCTGGATCAGCCCCTCATTGTGATCTGGGGTATGTG<\/p>\n

\u0413\u043b\u0430\u0432\u0430 13.<\/h2>\n

\u041f\u0440\u0430\u043a\u0442\u0438\u0447\u0435\u0441\u043a\u0430\u044f \u0440\u0430\u0431\u043e\u0442\u0430 1. \u041a\u041e\u041d\u0421\u0422\u0420\u0423\u0418\u0420\u041e\u0412\u0410\u041d\u0418\u0415 \u041f\u0420\u0410\u0419\u041c\u0415\u0420\u041e\u0412<\/p>\n

5\u2019-ATGTCGTGGGCCCTGGAAATGGCGGACACCTTCCTGGACAACATGCGGGTTGGGCCCAGGACGTACGCCGACGTACGCGATGAGATCAAT -3\u2019<\/p>\n

3\u2019-TACAGCACCCGGGACCTTTACCGCCTGTGGAAGGACCTGTTGTACGCCCAACCCGGGTCCTGCATGCGGCTGCATGCGCTACTCTAGTTA -5\u2019<\/p>\n

\u0413\u043b\u0430\u0432\u0430 17.<\/h2>\n

\u041f\u0440\u0430\u043a\u0442\u0438\u0447\u0435\u0441\u043a\u0430\u044f \u0440\u0430\u0431\u043e\u0442\u0430 7. \u00ab\u041a\u041e\u041d\u0421\u0422\u0420\u0423\u0418\u0420\u041e\u0412\u0410\u041d\u0418\u0415 \u041d\u0410\u041f\u0420\u0410\u0412\u041b\u042f\u042e\u0429\u0415\u0419\u00a0 \u0420\u041d\u041a \u0414\u041b\u042f \u0421\u0418\u0421\u0422\u0415\u041c\u042b CRISPR\/CAS9<\/p>\n

GTG-CCT-GCG-CTG-GAA-GCA-TGC-CCG-CAA-AAA-CGT-GGC-GTA-TGT-ACT-CGTGTA-TAT-ACT-ACC-ACC-CCT-A(A\/C)A-AAA-CCG-AAC-TCC-GCA-CTG-CGT-AAA-GTT-TGC-CGT-GTT-CGT-CTG-ACT-AAC-GGT<\/p>\n

ValProAlaLejGluAlaCisProGlnLizArgGliValCisTreArgValTirTreTreTreProLizLizProAsnSerAlaLejArgLizValCisArgValArgLejTreAsnGli<\/p>\n

5\u2019-GUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUU-3\u2019<\/p>\n

\u041b\u0430\u0431\u043e\u0440\u0430\u0442\u043e\u0440\u043d\u0430\u044f \u0440\u0430\u0431\u043e\u0442\u0430 14. \u00ab\u0422\u0415\u0421\u0422 \u041d\u0410 \u0413\u041c\u041e\u00bb<\/p>\n

1.<\/p>\n

5\u2019-CCCGACAGTGGTCCCAAAGATGGAC-3\u2019 (\u043f\u0435\u0440\u0432\u044b\u0439 \u043f\u0440\u044f\u043c\u043e\u0439 \u043f\u0440\u0430\u0439\u043c\u0435\u0440),<\/p>\n

5\u2019-ATATAGAGGAAGGGTCTTGCGAAGG-3\u2019 (\u043f\u0435\u0440\u0432\u044b\u0439 \u043e\u0431\u0440\u0430\u0442\u043d\u044b\u0439 \u043f\u0440\u0430\u0439\u043c\u0435\u0440).<\/p>\n

2.<\/p>\n

5\u2019-GGGATCCACTTCATGCTTTCGTCC-3\u2019 (\u0432\u0442\u043e\u0440\u043e\u0439 \u043f\u0440\u044f\u043c\u043e\u0439 \u043f\u0440\u0430\u0439\u043c\u0435\u0440),<\/p>\n

5\u2019-GGGAACCACATCACCACGGTACAT-3\u2019 (\u0432\u0442\u043e\u0440\u043e\u0439 \u043e\u0431\u0440\u0430\u0442\u043d\u044b\u0439 \u043f\u0440\u0430\u0439\u043c\u0435\u0440).<\/p>\n","protected":false},"excerpt":{"rendered":"

\u0413\u043b\u0430\u0432\u0430 5. \u0417\u0430\u0434\u0430\u0447\u0430 \u00ab\u0423\u0437\u043d\u0430\u0439, \u0447\u0442\u043e \u044d\u0442\u043e \u0437\u0430 \u0431\u0430\u043a\u0442\u0435\u0440\u0438\u044f, \u043f\u043e \u0414\u041d\u041a\u00bb GGGAGTAAAGTTAATACCTTTGCTC \u0413\u043b\u0430\u0432\u0430 7. \u0417\u0430\u0434\u0430\u0447\u0430 \u00ab\u0427\u0442\u043e \u0432\u044b\u043a\u043b\u044e\u0447\u0430\u0435\u0442 \u0420\u041d\u041a\u00bb AAUUGUUCCACAAUGCCACGC \u0413\u043b\u0430\u0432\u0430 11. \u0417\u0430\u0434\u0430\u0447\u0430 \u00ab\u041e \u0447\u0451\u043c \u043c\u043e\u0436\u0435\u0442 \u0441\u043a\u0430\u0437\u0430\u0442\u044c \u043d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434\u043d\u0430\u044f \u0437\u0430\u043c\u0435\u043d\u0430\u00bb \u041d\u043e\u0440\u043c\u0430 ACCTTGGCTGTACCCCCTGGGGAAGAGCAGAGATATACGTGCCAGGTGGAGCACCCAGGCCTGGATCAGCCCCTCATTGTGATCTGGGGTATGTG \u041f\u0430\u0446\u0438\u0435\u043d\u0442 ACCTTGGCTGTACCCCCTGGGGAAGAGCAGAGATATACGTACCAGGTGGAGCACCCAGGCCTGGATCAGCCCCTCATTGTGATCTGGGGTATGTG \u0413\u043b\u0430\u0432\u0430 13. \u041f\u0440\u0430\u043a\u0442\u0438\u0447\u0435\u0441\u043a\u0430\u044f \u0440\u0430\u0431\u043e\u0442\u0430 1. \u041a\u041e\u041d\u0421\u0422\u0420\u0423\u0418\u0420\u041e\u0412\u0410\u041d\u0418\u0415 \u041f\u0420\u0410\u0419\u041c\u0415\u0420\u041e\u0412 5\u2019-ATGTCGTGGGCCCTGGAAATGGCGGACACCTTCCTGGACAACATGCGGGTTGGGCCCAGGACGTACGCCGACGTACGCGATGAGATCAAT -3\u2019 3\u2019-TACAGCACCCGGGACCTTTACCGCCTGTGGAAGGACCTGTTGTACGCCCAACCCGGGTCCTGCATGCGGCTGCATGCGCTACTCTAGTTA -5\u2019 \u0413\u043b\u0430\u0432\u0430 17. \u041f\u0440\u0430\u043a\u0442\u0438\u0447\u0435\u0441\u043a\u0430\u044f \u0440\u0430\u0431\u043e\u0442\u0430 7. \u00ab\u041a\u041e\u041d\u0421\u0422\u0420\u0423\u0418\u0420\u041e\u0412\u0410\u041d\u0418\u0415 \u041d\u0410\u041f\u0420\u0410\u0412\u041b\u042f\u042e\u0429\u0415\u0419\u00a0 \u0420\u041d\u041a \u0414\u041b\u042f \u0421\u0418\u0421\u0422\u0415\u041c\u042b CRISPR\/CAS9 GTG-CCT-GCG-CTG-GAA-GCA-TGC-CCG-CAA-AAA-CGT-GGC-GTA-TGT-ACT-CGTGTA-TAT-ACT-ACC-ACC-CCT-A(A\/C)A-AAA-CCG-AAC-TCC-GCA-CTG-CGT-AAA-GTT-TGC-CGT-GTT-CGT-CTG-ACT-AAC-GGT ValProAlaLejGluAlaCisProGlnLizArgGliValCisTreArgValTirTreTreTreProLizLizProAsnSerAlaLejArgLizValCisArgValArgLejTreAsnGli … \u0427\u0438\u0442\u0430\u0442\u044c \u0434\u0430\u043b\u0435\u0435 \u041d\u0430\u0431\u043e\u0440\u044b \u0434\u0430\u043d\u043d\u044b\u0445 \u0438\u00a0 \u043f\u043e\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u0442\u0435\u043b\u044c\u043d\u043e\u0441\u0442\u0438 \u0434\u043b\u044f \u043f\u0440\u0430\u043a\u0442\u0438\u0447\u0435\u0441\u043a\u0438\u0445 \u0440\u0430\u0431\u043e\u0442<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/tutorial\/wp-json\/wp\/v2\/pages\/56"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/tutorial\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/tutorial\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/tutorial\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/tutorial\/wp-json\/wp\/v2\/comments?post=56"}],"version-history":[{"count":1,"href":"https:\/\/sites.icgbio.ru\/tutorial\/wp-json\/wp\/v2\/pages\/56\/revisions"}],"predecessor-version":[{"id":57,"href":"https:\/\/sites.icgbio.ru\/tutorial\/wp-json\/wp\/v2\/pages\/56\/revisions\/57"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/tutorial\/wp-json\/wp\/v2\/media?parent=56"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}