{"id":137,"date":"2020-08-09T02:30:47","date_gmt":"2020-08-09T05:30:47","guid":{"rendered":"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/?p=137"},"modified":"2020-11-06T15:15:48","modified_gmt":"2020-11-06T18:15:48","slug":"endothelial-differentiation","status":"publish","type":"post","link":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/endothelial-differentiation\/","title":{"rendered":"Endothelial differentiation"},"content":{"rendered":"<p><a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Endothelial-differentiation-.jpg\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-473\" src=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Endothelial-differentiation-.jpg\" alt=\"\" width=\"1280\" height=\"1024\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Endothelial-differentiation-.jpg 1280w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Endothelial-differentiation--300x240.jpg 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Endothelial-differentiation--700x560.jpg 700w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Endothelial-differentiation--768x614.jpg 768w\" sizes=\"(max-width: 1280px) 100vw, 1280px\" \/><\/a><\/p>\n<p align=\"justify\">iPSC-derived endothelial cells serve as a convenient model to study molecular mechanisms of vascular diseases, provide an opportunity for screening of novel drugs, creating of vascularized 3D-organoids and vascular regeneration. We generate endothelial cells from human PSCs using a monolayer differentiation approach (Gu, 2018, doi: 10.1002\/cphg.64 with changes). The first step is efficiently mesoderm generation via small\u2010molecule activation of WNT signaling for 4 days. A subsequent endothelial cells specification is carried out for another 4 days through specific growth factors. After 8 days of differentiation, mature endothelial cells are enriched by magnetic\u2010activated cell sorting for the surface marker CD31. The obtained endothelial cells express of specific markers: CD 31, vWF, VEGFR2, ANG1, bFGF, CXCR4, SDF1, form of tube\u2010like structures and uptake of acetylate low\u2010density lipoprotein.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>iPSC-derived endothelial cells serve as a convenient model to study molecular mechanisms of vascular diseases, provide an opportunity for screening of novel drugs, creating of vascularized 3D-organoids and vascular regeneration. We generate endothelial cells from human PSCs using a monolayer differentiation approach (Gu, 2018, doi: 10.1002\/cphg.64 with changes). The first step is efficiently mesoderm generation [&hellip;]<\/p>\n","protected":false},"author":31,"featured_media":473,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[43],"tags":[33,37],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/137"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/comments?post=137"}],"version-history":[{"count":8,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/137\/revisions"}],"predecessor-version":[{"id":613,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/137\/revisions\/613"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media\/473"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media?parent=137"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/categories?post=137"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/tags?post=137"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}