{"id":140,"date":"2020-08-09T02:32:47","date_gmt":"2020-08-09T05:32:47","guid":{"rendered":"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/?p=140"},"modified":"2020-11-06T15:14:58","modified_gmt":"2020-11-06T18:14:58","slug":"hepatocyte-differentiation","status":"publish","type":"post","link":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/hepatocyte-differentiation\/","title":{"rendered":"Hepatocyte differentiation"},"content":{"rendered":"<p align=\"justify\"><a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation2.jpg\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-474\" src=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation2.jpg\" alt=\"\" width=\"1280\" height=\"1024\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation2.jpg 1280w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation2-300x240.jpg 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation2-700x560.jpg 700w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation2-768x614.jpg 768w\" sizes=\"(max-width: 1280px) 100vw, 1280px\" \/><\/a><\/p>\n<p align=\"justify\">iPSC-derived hepatocyte-like cells are a convenient tool for hepatotoxic screening of potential medical products, basic research of liver diseases pathogenesis, and for the development of cell technologies for the liver diseases treatment. We used a monolayer differentiation protocol to obtain hepatocyte-like cells from human iPSCs using specific growth factors (Tasnim et al., 2015, doi: 10.1016\/j.biomaterials.2015.08.002). During 6 days of the differentiation iPSCs were converted into a definitive endoderm by the activation of Nodal and Wnt signaling pathways. At this stage, specific factors (GATA4, HNF4A, HNF3B \/ FOXA2, TBX3) were expressed. At the next stage, there was a transition to hepatoblasts for one week (SOX17, AFP, ALBUMIN). At the terminal stages of differentiation hepatocytes expressed AAT, CYP1A1, TRANSFERRIN.<\/p>\n<p align=\"justify\"><a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation1.jpg\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-475\" src=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation1.jpg\" alt=\"\" width=\"1280\" height=\"1024\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation1.jpg 1280w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation1-300x240.jpg 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation1-700x560.jpg 700w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Hepatocyte-differentiation1-768x614.jpg 768w\" sizes=\"(max-width: 1280px) 100vw, 1280px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>iPSC-derived hepatocyte-like cells are a convenient tool for hepatotoxic screening of potential medical products, basic research of liver diseases pathogenesis, and for the development of cell technologies for the liver diseases treatment. We used a monolayer differentiation protocol to obtain hepatocyte-like cells from human iPSCs using specific growth factors (Tasnim et al., 2015, doi: 10.1016\/j.biomaterials.2015.08.002). [&hellip;]<\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"gallery","meta":[],"categories":[43],"tags":[33,38],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/140"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/comments?post=140"}],"version-history":[{"count":9,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/140\/revisions"}],"predecessor-version":[{"id":609,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/140\/revisions\/609"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media?parent=140"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/categories?post=140"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/tags?post=140"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}