{"id":150,"date":"2020-08-09T03:04:01","date_gmt":"2020-08-09T06:04:01","guid":{"rendered":"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/?p=150"},"modified":"2020-11-06T15:19:11","modified_gmt":"2020-11-06T18:19:11","slug":"motor-neurons","status":"publish","type":"post","link":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/motor-neurons\/","title":{"rendered":"Motor neurons"},"content":{"rendered":"<p><a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Motor-neurons-1.jpg\" rel=\"noopener noreferrer\"><img loading=\"lazy\" class=\"alignnone wp-image-418 size-full\" title=\"Differentiation Motor neurons \" src=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Motor-neurons-1.jpg\" alt=\"Differentiation-Motor-neurons\" width=\"1280\" height=\"1024\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Motor-neurons-1.jpg 1280w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Motor-neurons-1-300x240.jpg 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Motor-neurons-1-700x560.jpg 700w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/08\/Differentiation-Motor-neurons-1-768x614.jpg 768w\" sizes=\"(max-width: 1280px) 100vw, 1280px\" \/><\/a><\/p>\n<p align=\"justify\">Spinal motor neurons (MNs) are a highly specialized type of neurons that reside in the ventral horns and project axons to muscles to control their movement. Degeneration of MNs is implicated in a number of devastating diseases, including spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS), Charcot-Marie-Tooth and poliomyelitis disease. The above disease iPSCs have been generated from patients and attempts have been made to identify disease-related phenotypes and to dissect out the underlying mechanisms before embarking on drug discovery. using a combination of small molecules in a chemical defined neural medium, we have guided hPSCs to a near-pure population of OLIG2 \u00fe MNPs in 12 days, and a highly enriched population of functionally mature MNs (490%) in another 16 days by the use of a Notch inhibitor. Furthermore, our approach enables expanding a single MNP to 1 104 MNPs, producing a large quantity (5 105) of consistent MNs from a single stem cell. MNs generated by this method display molecular phenotypes of SMA and ALS, and can be readily adapted to screening platforms.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Spinal motor neurons (MNs) are a highly specialized type of neurons that reside in the ventral horns and project axons to muscles to control their movement. Degeneration of MNs is implicated in a number of devastating diseases, including spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS), Charcot-Marie-Tooth and poliomyelitis disease. The above disease iPSCs have [&hellip;]<\/p>\n","protected":false},"author":31,"featured_media":418,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[43,23],"tags":[33,40,35],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/150"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/comments?post=150"}],"version-history":[{"count":32,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/150\/revisions"}],"predecessor-version":[{"id":224,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/150\/revisions\/224"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media\/418"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media?parent=150"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/categories?post=150"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/tags?post=150"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}