{"id":2425,"date":"2022-07-08T06:31:09","date_gmt":"2022-07-08T09:31:09","guid":{"rendered":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/?p=2425"},"modified":"2022-07-08T07:30:44","modified_gmt":"2022-07-08T10:30:44","slug":"familial-hypercholesterolemia","status":"publish","type":"post","link":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/familial-hypercholesterolemia\/","title":{"rendered":"Familial hypercholesterolemia"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2425\" class=\"elementor elementor-2425\">\n\t\t\t\t\t\t<div class=\"elementor-inner\">\n\t\t\t\t<div class=\"elementor-section-wrap\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fdd3728 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fdd3728\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f32cf56\" data-id=\"f32cf56\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2e7f6bd elementor-widget elementor-widget-text-editor\" data-id=\"2e7f6bd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Familial hypercholesterolemia (FH) is a monogenic disease, leading to atherosclerosis due to a high level of low-density lipoprotein cholesterol. Most cases of the disease are based on pathological variants in the <em>LDLR <\/em>gene<em>. <\/em>Available treatments are effective not for all LDLR mutations. The development of cellular models for FH is an important direction for creating new approaches to atherosclerosis treatment.<\/p><p>This study was supported by the Russian Science Foundation (grant \u2116 21-15-00065).<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-404acaba elementor-hidden-mobile elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"404acaba\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-2049a245\" data-id=\"2049a245\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2f6c5d73 elementor-widget elementor-widget-image\" data-id=\"2f6c5d73\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"Diseased IPSC Lines-Familial Hypercholesterolemia\" data-elementor-lightbox-description=\"Characterization of the ICGi036-A iPSC line.\nA. Morphology of the iPSC colonies. \nB. Immunofluorescent staining for pluripotency markers OCT4, NANOG,TRA-1-60, SSEA4. \nC. Mutation analysis. Sanger sequencing of exons 7 and 4 of LDLR in the parental PBMCs and ICGi036-A iPSC line. Nucleotide substitutions areshown with red rectangles. \nD. Analysis of pluripotency genes (OCT4, NANOG, SOX2) expression in the iPSC line relative to parental PBMCs by RT-qPCR. Humanembryonic stem cell line, HUES9, was used as a control. \nE. Karyotype of the iPSC line. \nF. Immunofluorescent staining for three germ layer markers, \u03b1SMA andcollagen type I (mesoderm), NF200 and TUBB3 (ectoderm), CK18 and FOXA2\/HNF3\u03b2 (endoderm), after spontaneous in vitro differentiation in embryoid bodies.\n\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjI0MzQiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMlwvMDdcL0lQU0MtRGlzZWFzZWQtSVBTQy1MaW5lcy1GYW1pbGlhbC1IeXBlcmNob2xlc3Rlcm9sZW1pYV8xLnBuZyJ9\">\n\t\t\t\t\t\t\t<img width=\"1200\" height=\"1636\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1.png\" class=\"attachment-full size-full\" alt=\"Diseased IPSC Lines-Familial Hypercholesterolemia_1\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1-768x1047.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1-1127x1536.png 1127w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-2495ce64\" data-id=\"2495ce64\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-19a897b4 elementor-widget elementor-widget-image\" data-id=\"19a897b4\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"Diseased IPSC Lines-Familial Hypercholesterolemia_2\" data-elementor-lightbox-description=\"Characterization of the ICGi037-A iPSC line. \nA. Morphology of the iPSC colonies.\nB. Immunofluorescent staining for pluripotency markers OCT4, NANOG,TRA-1-60, SSEA4. \nC. Analysis of pluripotency genes (OCT4, NANOG, SOX2) expression in the iPSC line relative to parental PBMCs by RT-qPCR. Human embryonicstem cell line, HUES9, was used as a control. \nD. Mutation analysis. Illumina HiSeq1500 sequencing of LDLR exon 9 in the ICGi037-A iPSC line. Nucleotide substitutionsare shown with red rectangles. \nE. Karyotype of the iPSC line. \nF. Immunofluorescent staining for three germ layer markers, \u03b1SMA and CD90 (mesoderm), TUBB3 and GFAP (ectoderm), CK18 and FOXA2\/HNF3\u03b2 (endoderm), after spontaneous in vitro differentiation in embryoid bodies.\n\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjI0NDIiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMlwvMDdcL0lQU0MtRGlzZWFzZWQtSVBTQy1MaW5lcy1GYW1pbGlhbC1IeXBlcmNob2xlc3Rlcm9sZW1pYV8yLnBuZyJ9\">\n\t\t\t\t\t\t\t<img width=\"1200\" height=\"1636\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2.png\" class=\"attachment-full size-full\" alt=\"Diseased IPSC Lines-Familial Hypercholesterolemia\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2-768x1047.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2-1127x1536.png 1127w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-1ee0d3f6\" data-id=\"1ee0d3f6\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-29cf25fb elementor-widget elementor-widget-image\" data-id=\"29cf25fb\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"Diseased IPSC Lines-Familial Hypercholesterolemia\" data-elementor-lightbox-description=\"Characterization of the ICGi038-A iPSC line. \nA. Morphology of the iPSC colonies. \nB. Immunofluorescent staining for pluripotency markers OCT4, NANOG, TRA-1-60, SSEA4. \nC. Analysis of pluripotency genes (OCT4, NANOG, SOX2) expression in the iPSC line relative to parental PBMCs by RT-qPCR. Human embryonicstem cell line, HUES9, was used as a control. \nD. Mutation analysis. Illumina HiSeq1500 sequencing of LDLR in the ICGi038-A iPSC line. Nucleotide substitutions areshown with red rectangles. \nE. Karyotype of the iPSC line. \nF. Immunofluorescent staining for three germ layer markers, \u03b1SMA and CD90 (mesoderm), TUBB3 andNF200 (ectoderm), FOXA2\/HNF3\u03b2 and CK18 (endoderm), after spontaneous in vitro differentiation in embryoid bodies.\n\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjI0NDYiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMlwvMDdcL0lQU0MtRGlzZWFzZWQtSVBTQy1MaW5lcy1GYW1pbGlhbC1IeXBlcmNob2xlc3Rlcm9sZW1pYV8zLnBuZyJ9\">\n\t\t\t\t\t\t\t<img width=\"1200\" height=\"1636\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3.png\" class=\"attachment-full size-full\" alt=\"IPSC-Diseased IPSC Lines-Familial Hypercholesterolemia\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3-768x1047.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3-1127x1536.png 1127w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e8effcb elementor-hidden-desktop elementor-hidden-tablet elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e8effcb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-1b62b0c\" data-id=\"1b62b0c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9f4a7ba elementor-widget elementor-widget-image\" data-id=\"9f4a7ba\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img width=\"1200\" height=\"1636\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1.png\" class=\"attachment-full size-full\" alt=\"Diseased IPSC Lines-Familial Hypercholesterolemia_1\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1-768x1047.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_1-1127x1536.png 1127w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Characterization of the ICGi036-A iPSC line. A. Morphology of the iPSC colonies.  B. Immunofluorescent staining for pluripotency markers OCT4, NANOG,TRA-1-60, SSEA4.  C. Mutation analysis. Sanger sequencing of exons 7 and 4 of LDLR in the parental PBMCs and ICGi036-A iPSC line. Nucleotide substitutions areshown with red rectangles.  D. Analysis of pluripotency genes (OCT4, NANOG, SOX2) expression in the iPSC line relative to parental PBMCs by RT-qPCR. Humanembryonic stem cell line, HUES9, was used as a control.  E. Karyotype of the iPSC line.  F. Immunofluorescent staining for three germ layer markers, \u03b1SMA andcollagen type I (mesoderm), NF200 and TUBB3 (ectoderm), CK18 and FOXA2\/HNF3\u03b2 (endoderm), after spontaneous in vitro differentiation in embryoid bodies.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-e092486\" data-id=\"e092486\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6ad9c05 elementor-widget elementor-widget-image\" data-id=\"6ad9c05\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img width=\"1200\" height=\"1636\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2.png\" class=\"attachment-full size-full\" alt=\"Diseased IPSC Lines-Familial Hypercholesterolemia\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2-768x1047.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_2-1127x1536.png 1127w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Characterization of the ICGi037-A iPSC line.  A. Morphology of the iPSC colonies. B. Immunofluorescent staining for pluripotency markers OCT4, NANOG,TRA-1-60, SSEA4.  C. Analysis of pluripotency genes (OCT4, NANOG, SOX2) expression in the iPSC line relative to parental PBMCs by RT-qPCR. Human embryonicstem cell line, HUES9, was used as a control.  D. Mutation analysis. Illumina HiSeq1500 sequencing of LDLR exon 9 in the ICGi037-A iPSC line. Nucleotide substitutionsare shown with red rectangles.  E. Karyotype of the iPSC line.  F. Immunofluorescent staining for three germ layer markers, \u03b1SMA and CD90 (mesoderm), TUBB3 and GFAP (ectoderm), CK18 and FOXA2\/HNF3\u03b2 (endoderm), after spontaneous in vitro differentiation in embryoid bodies.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-8238661\" data-id=\"8238661\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e96b93b elementor-widget elementor-widget-image\" data-id=\"e96b93b\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img width=\"1200\" height=\"1636\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3.png\" class=\"attachment-full size-full\" alt=\"IPSC-Diseased IPSC Lines-Familial Hypercholesterolemia\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3-768x1047.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2022\/07\/IPSC-Diseased-IPSC-Lines-Familial-Hypercholesterolemia_3-1127x1536.png 1127w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Characterization of the ICGi038-A iPSC line.  A. Morphology of the iPSC colonies.  B. Immunofluorescent staining for pluripotency markers OCT4, NANOG,TRA-1-60, SSEA4.  C. Analysis of pluripotency genes (OCT4, NANOG, SOX2) expression in the iPSC line relative to parental PBMCs by RT-qPCR. Human embryonicstem cell line, HUES9, was used as a control.  D. Mutation analysis. Illumina HiSeq1500 sequencing of LDLR in the ICGi038-A iPSC line. Nucleotide substitutions areshown with red rectangles.  E. Karyotype of the iPSC line.  F. Immunofluorescent staining for three germ layer markers, \u03b1SMA and CD90 (mesoderm), TUBB3 andNF200 (ectoderm), FOXA2\/HNF3\u03b2 and CK18 (endoderm), after spontaneous in vitro differentiation in embryoid bodies.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Familial hypercholesterolemia (FH) is a monogenic disease, leading to atherosclerosis due to a high level of low-density lipoprotein cholesterol. Most cases of the disease are based on pathological variants in the LDLR gene. Available treatments are effective not for all LDLR mutations. The development of cellular models for FH is an important direction for creating [&hellip;]<\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":[],"categories":[2],"tags":[59,71],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/2425"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/comments?post=2425"}],"version-history":[{"count":19,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/2425\/revisions"}],"predecessor-version":[{"id":2456,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/2425\/revisions\/2456"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media?parent=2425"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/categories?post=2425"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/tags?post=2425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}