{"id":501,"date":"2020-09-18T07:58:12","date_gmt":"2020-09-18T10:58:12","guid":{"rendered":"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/?p=501"},"modified":"2020-12-04T02:07:48","modified_gmt":"2020-12-04T05:07:48","slug":"isogenic-lines-constructions","status":"publish","type":"post","link":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/isogenic-lines-constructions\/","title":{"rendered":"Isogenic lines constructions"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"501\" class=\"elementor elementor-501\">\n\t\t\t\t\t\t<div class=\"elementor-inner\">\n\t\t\t\t<div class=\"elementor-section-wrap\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-51054c6 elementor-hidden-phone elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"51054c6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-357a4c20\" data-id=\"357a4c20\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7fc314a elementor-widget elementor-widget-image\" data-id=\"7fc314a\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"Plasmid constructions Isogenic lines constructions\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MTY2MCwidXJsIjoiaHR0cHM6XC9cL3NpdGVzLmljZ2Jpby5ydVwvemFraWFubGFiLWlwc2Njb2xsZWN0aW9uXC93cC1jb250ZW50XC91cGxvYWRzXC9zaXRlc1wvMzFcLzIwMjBcLzA5XC9QbGFzbWlkLWNvbnN0cnVjdGlvbnMtSXNvZ2VuaWMtbGluZXMtY29uc3RydWN0aW9ucy5wbmcifQ%3D%3D\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions.png\" class=\"attachment-full size-full\" alt=\"Plasmid constructions Isogenic lines constructions\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6c04029e elementor-widget elementor-widget-text-editor\" data-id=\"6c04029e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid for homologous recombination in the <i>HTT<\/i> locus for Huntington&#8217;s disease modeling. The plasmid contains long homology arms to the first exon of <i>HTT<\/i>, which flank 215 CAG repeats. We have a set of identical donor plasmids differing\u00a0from each other by the number of CAG triplets.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-54fe1de5\" data-id=\"54fe1de5\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-3eb31fd0\" data-id=\"3eb31fd0\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-77791be elementor-hidden-desktop elementor-hidden-tablet elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"77791be\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-8170961\" data-id=\"8170961\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7061aa1 elementor-widget elementor-widget-image\" data-id=\"7061aa1\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions.png\" class=\"attachment-full size-full\" alt=\"Plasmid constructions Isogenic lines constructions\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Plasmid-constructions-Isogenic-lines-constructions-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f18869c elementor-widget elementor-widget-text-editor\" data-id=\"f18869c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid for homologous recombination in the <i>HTT<\/i> locus for Huntington&#8217;s disease modeling. The plasmid contains long homology arms to the first exon of <i>HTT<\/i>, which flank 215 CAG repeats. We have a set of identical donor plasmids differing\u00a0from each other by the number of CAG triplets.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-937fc06\" data-id=\"937fc06\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-bd293ba\" data-id=\"bd293ba\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Donor plasmid for homologous recombination in the HTT locus for Huntington&#8217;s disease modeling. The plasmid contains long homology arms to the first exon of HTT, which flank 215 CAG repeats. We have a set of identical donor plasmids differing\u00a0from each other by the number of CAG triplets. Donor plasmid for homologous recombination in the HTT [&hellip;]<\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":[],"categories":[47],"tags":[45,46],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/501"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/comments?post=501"}],"version-history":[{"count":20,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/501\/revisions"}],"predecessor-version":[{"id":1836,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/501\/revisions\/1836"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media?parent=501"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/categories?post=501"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/tags?post=501"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}