{"id":505,"date":"2020-09-18T08:10:44","date_gmt":"2020-09-18T11:10:44","guid":{"rendered":"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/?p=505"},"modified":"2024-08-02T17:32:38","modified_gmt":"2024-08-02T20:32:38","slug":"protein-protein-interactions","status":"publish","type":"post","link":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/protein-protein-interactions\/","title":{"rendered":"Protein-protein interactions"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"505\" class=\"elementor elementor-505\">\n\t\t\t\t\t\t<div class=\"elementor-inner\">\n\t\t\t\t<div class=\"elementor-section-wrap\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e9061b7 elementor-hidden-tablet elementor-hidden-phone elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e9061b7\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d0dcede\" data-id=\"d0dcede\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4e80a4a elementor-widget elementor-widget-spacer\" data-id=\"4e80a4a\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1706e2b9 elementor-hidden-phone elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1706e2b9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-5fbcab3\" data-id=\"5fbcab3\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3544f93 elementor-widget elementor-widget-heading\" data-id=\"3544f93\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3beta-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-70cd863 elementor-widget elementor-widget-image\" data-id=\"70cd863\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-14-3-3beta-C-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjEwOTYiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS0xNC0zLTNiZXRhLUMtM1hGTEFHLTJYU1QucG5nIn0%3D\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-629ea3c elementor-widget elementor-widget-text-editor\" data-id=\"629ea3c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3beta protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-7e52de14\" data-id=\"7e52de14\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f3d8d06 elementor-widget elementor-widget-heading\" data-id=\"f3d8d06\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3epsilon-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5e6afeca elementor-widget elementor-widget-image\" data-id=\"5e6afeca\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-14-3-3epsilon-C-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjEwOTciLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS0xNC0zLTNlcHNpbG9uLUMtM1hGTEFHLTJYU1QucG5nIn0%3D\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3epsilon-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d6704c6 elementor-widget elementor-widget-text-editor\" data-id=\"d6704c6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3epsilon protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-de76896\" data-id=\"de76896\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-59c3ed3 elementor-widget elementor-widget-heading\" data-id=\"59c3ed3\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3eta-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2493746 elementor-widget elementor-widget-image\" data-id=\"2493746\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-14-3-3eta-C-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjExMDAiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS0xNC0zLTNldGEtQy0zWEZMQUctMlhTVC5wbmcifQ%3D%3D\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3eta-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8646819 elementor-widget elementor-widget-text-editor\" data-id=\"8646819\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3eta protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-697d6b7 elementor-hidden-desktop elementor-hidden-tablet elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"697d6b7\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-9685919\" data-id=\"9685919\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f5021aa elementor-widget elementor-widget-heading\" data-id=\"f5021aa\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3beta-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8eb53e5 elementor-widget elementor-widget-image\" data-id=\"8eb53e5\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3beta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-676317e elementor-widget elementor-widget-text-editor\" data-id=\"676317e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3beta protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-dbdbb72\" data-id=\"dbdbb72\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7d46d8f elementor-widget elementor-widget-heading\" data-id=\"7d46d8f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3epsilon-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-042ba85 elementor-widget elementor-widget-image\" data-id=\"042ba85\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3epsilon-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3epsilon-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fddfdf4 elementor-widget elementor-widget-text-editor\" data-id=\"fddfdf4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3epsilon protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ead7a8b elementor-hidden-phone elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ead7a8b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-2fcd61a\" data-id=\"2fcd61a\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7d0052c elementor-widget elementor-widget-heading\" data-id=\"7d0052c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3gamma-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7b4d06d elementor-widget elementor-widget-image\" data-id=\"7b4d06d\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-14-3-3gamma-C-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjExMDEiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS0xNC0zLTNnYW1tYS1DLTNYRkxBRy0yWFNULnBuZyJ9\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3gamma-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-911a7c8 elementor-widget elementor-widget-text-editor\" data-id=\"911a7c8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3gamma protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-62b2a96\" data-id=\"62b2a96\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-98f5361 elementor-widget elementor-widget-heading\" data-id=\"98f5361\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3sigma-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ec6e096 elementor-widget elementor-widget-image\" data-id=\"ec6e096\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-14-3-3sigma-C-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjExMDIiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS0xNC0zLTNzaWdtYS1DLTNYRkxBRy0yWFNULnBuZyJ9\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dbbd23d elementor-widget elementor-widget-text-editor\" data-id=\"dbbd23d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3sigma protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-2dcf637\" data-id=\"2dcf637\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bf7d4ed elementor-widget elementor-widget-heading\" data-id=\"bf7d4ed\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3theta-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-57bad2e elementor-widget elementor-widget-image\" data-id=\"57bad2e\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-14-3-3theta-C-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjExMDUiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS0xNC0zLTN0aGV0YS1DLTNYRkxBRy0yWFNULnBuZyJ9\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3theta-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ffaaebd elementor-widget elementor-widget-text-editor\" data-id=\"ffaaebd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3theta protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7e56ef0 elementor-hidden-desktop elementor-hidden-tablet elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7e56ef0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-ec1d649\" data-id=\"ec1d649\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f0f0b8f elementor-widget elementor-widget-heading\" data-id=\"f0f0b8f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3eta-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6255540 elementor-widget elementor-widget-image\" data-id=\"6255540\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3eta-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3eta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fb20fab elementor-widget elementor-widget-text-editor\" data-id=\"fb20fab\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3eta protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-9c953e2\" data-id=\"9c953e2\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f80d2d4 elementor-widget elementor-widget-heading\" data-id=\"f80d2d4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3gamma-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2980209 elementor-widget elementor-widget-image\" data-id=\"2980209\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3gamma-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3gamma-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-974676c elementor-widget elementor-widget-text-editor\" data-id=\"974676c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3gamma protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-9cf0c87\" data-id=\"9cf0c87\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-64acf0b elementor-widget elementor-widget-heading\" data-id=\"64acf0b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3sigma-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8fdbd71 elementor-widget elementor-widget-image\" data-id=\"8fdbd71\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3sigma-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d0b557c elementor-widget elementor-widget-text-editor\" data-id=\"d0b557c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3sigma protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-69a2b85 elementor-hidden-phone elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"69a2b85\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-32d4797\" data-id=\"32d4797\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6bdb175 elementor-widget elementor-widget-heading\" data-id=\"6bdb175\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3zeta-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f6aef83 elementor-widget elementor-widget-image\" data-id=\"f6aef83\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-14-3-3zeta-C-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjExMDYiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS0xNC0zLTN6ZXRhLUMtM1hGTEFHLTJYU1QucG5nIn0%3D\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3zeta-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3f800a7 elementor-widget elementor-widget-text-editor\" data-id=\"3f800a7\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3zeta protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-abcdce4\" data-id=\"abcdce4\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a597af8 elementor-widget elementor-widget-heading\" data-id=\"a597af8\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-TagGFP2-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2562100 elementor-widget elementor-widget-image\" data-id=\"2562100\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-TagGFP2-C-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjExMDciLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS1UYWdHRlAyLUMtM1hGTEFHLTJYU1QucG5nIn0%3D\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-TagGFP2-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-86a25c1 elementor-widget elementor-widget-text-editor\" data-id=\"86a25c1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the TagGFP2 protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-465848e\" data-id=\"465848e\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-aa933d1 elementor-widget elementor-widget-heading\" data-id=\"aa933d1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-hMEK1-N-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d82e49e elementor-widget elementor-widget-image\" data-id=\"d82e49e\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"pAAVS1-hMEK1-N-3XFLAG-2XST\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjExMTEiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL3BBQVZTMS1oTUVLMS1OLTNYRkxBRy0yWFNULnBuZyJ9\">\n\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5c6f68c elementor-widget elementor-widget-text-editor\" data-id=\"5c6f68c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human MEK1 protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on N terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5c99ddd elementor-hidden-desktop elementor-hidden-tablet elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5c99ddd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-476a646\" data-id=\"476a646\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7dde595 elementor-widget elementor-widget-heading\" data-id=\"7dde595\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3theta-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0182820 elementor-widget elementor-widget-image\" data-id=\"0182820\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3theta-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3theta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d0300e2 elementor-widget elementor-widget-text-editor\" data-id=\"d0300e2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3theta protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-d1aee20\" data-id=\"d1aee20\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-66e713c elementor-widget elementor-widget-heading\" data-id=\"66e713c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-14-3-3zeta-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bb5a0ee elementor-widget elementor-widget-image\" data-id=\"bb5a0ee\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3zeta-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-72a194a elementor-widget elementor-widget-text-editor\" data-id=\"72a194a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human 14-3-3zeta protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-454418f\" data-id=\"454418f\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2267993 elementor-widget elementor-widget-heading\" data-id=\"2267993\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-TagGFP2-C-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a3e50c1 elementor-widget elementor-widget-image\" data-id=\"a3e50c1\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-TagGFP2-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ebaa690 elementor-widget elementor-widget-text-editor\" data-id=\"ebaa690\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the TagGFP2 protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e9b23ca elementor-hidden-desktop elementor-hidden-tablet elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e9b23ca\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-e5e5256\" data-id=\"e5e5256\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-aee1765 elementor-widget elementor-widget-heading\" data-id=\"aee1765\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">pAAVS1-hMEK1-N-3XFLAG-2XST<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3587a30 elementor-widget elementor-widget-image\" data-id=\"3587a30\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-hMEK1-N-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6a3c9fe elementor-widget elementor-widget-text-editor\" data-id=\"6a3c9fe\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. hPGK promoter driven expression of the human MEK1 protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on N terminus<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-b79c82c elementor-hidden-phone\" data-id=\"b79c82c\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3cb4200 elementor-widget elementor-widget-image\" data-id=\"3cb4200\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-14-3-3zeta-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-14-3-3zeta-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-e7fd84d elementor-hidden-phone\" data-id=\"e7fd84d\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b7b4f96 elementor-widget elementor-widget-image\" data-id=\"b7b4f96\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1180\" height=\"870\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST.png\" class=\"attachment-full size-full\" alt=\"pAAVS1-TagGFP2-C-3XFLAG-2XST\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST.png 1180w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-300x221.png 300w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-1024x755.png 1024w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/pAAVS1-TagGFP2-C-3XFLAG-2XST-768x566.png 768w\" sizes=\"(max-width: 1180px) 100vw, 1180px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c7576bd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c7576bd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-a60a62a\" data-id=\"a60a62a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f43271c elementor-widget elementor-widget-spacer\" data-id=\"f43271c\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the human alpha-synuclein protein with 3\u00d7FLAG and 2\u00d7Strep-Tag II tags on C terminus<\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":[],"categories":[47],"tags":[46,48],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/505"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/comments?post=505"}],"version-history":[{"count":47,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/505\/revisions"}],"predecessor-version":[{"id":2524,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/505\/revisions\/2524"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media?parent=505"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/categories?post=505"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/tags?post=505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}