{"id":522,"date":"2020-09-18T08:48:22","date_gmt":"2020-09-18T11:48:22","guid":{"rendered":"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/?p=522"},"modified":"2020-12-02T18:08:08","modified_gmt":"2020-12-02T21:08:08","slug":"huntingtons-disease_","status":"publish","type":"post","link":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/huntingtons-disease_\/","title":{"rendered":"Huntington\u2019s disease"},"content":{"rendered":"\t\t
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<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\"Isogenic\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Isogenic HD model<\/strong>
Huntington\u2019s disease (HD) is a severe neurodegenerative disorder caused by a CAG triplet expansion in the first exon of the HTT<\/em> gene. Here we generated isogenic iPSC-based HD model by introducing 69 CAG triplets into HTT<\/em> through CRISPR\/Cas9-mediated homologous recombination. The obtained isogenic cell system can serve as a valuable model for the investigation into various parameters of HD and for screening of potential drugs without worries about background-related variability.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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(A) The scheme of homologous recombination between wild-type HTT and the donor construct. HTT-F and HTT-R: primers for the detection of the insertion of the expanded CAG repeat tract into HTT; (B) PCR analysis of HTT allele lengths in the mutant cell clones; (C) Western blot analysis of HTT expression in the mutant cells; (D) Fragment analysis of PCR products by capillary electrophoresis. Green squares and arrows indicate the number of CAG repeats in the HTT alleles; (E) Immunofluorescence staining of isogenic HD iPSCs with antibodies against transcription factors OCT3\/4, NANOG, and SOX2 and surface antigen TRA-1-60; (F) Immunofluorescence staining confirming the expression of markers of three germ layers\u2019 derivatives after spontaneous differentiation of isogenic HD iPSCs. Scale bars: 100 \u00b5m.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Isogenic HD model<\/strong>
Huntington\u2019s disease (HD) is a severe neurodegenerative disorder caused by a CAG triplet expansion in the first exon of the HTT<\/em> gene. Here we generated isogenic iPSC-based HD model by introducing 69 CAG triplets into HTT<\/em> through CRISPR\/Cas9-mediated homologous recombination. The obtained isogenic cell system can serve as a valuable model for the investigation into various parameters of HD and for screening of potential drugs without worries about background-related variability.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Isogenic HD modelHuntington\u2019s disease (HD) is a severe neurodegenerative disorder caused by a CAG triplet expansion in the first exon of the HTT gene. Here we generated isogenic iPSC-based HD model by introducing 69 CAG triplets into HTT through CRISPR\/Cas9-mediated homologous recombination. The obtained isogenic cell system can serve as a valuable model for the […]<\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":[],"categories":[15],"tags":[56,54],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/522"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/comments?post=522"}],"version-history":[{"count":11,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/522\/revisions"}],"predecessor-version":[{"id":1804,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/522\/revisions\/1804"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media?parent=522"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/categories?post=522"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/tags?post=522"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}