{"id":551,"date":"2020-09-18T09:18:27","date_gmt":"2020-09-18T12:18:27","guid":{"rendered":"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/?p=551"},"modified":"2022-07-08T06:22:41","modified_gmt":"2022-07-08T09:22:41","slug":"huntingtons-disease","status":"publish","type":"post","link":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/huntingtons-disease\/","title":{"rendered":"Huntington\u2019s disease"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"551\" class=\"elementor elementor-551\">\n\t\t\t\t\t\t<div class=\"elementor-inner\">\n\t\t\t\t<div class=\"elementor-section-wrap\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-32fdb72b elementor-hidden-desktop elementor-hidden-tablet elementor-hidden-phone elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"32fdb72b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-79b5565e\" data-id=\"79b5565e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4f2e276 elementor-widget elementor-widget-spacer\" data-id=\"4f2e276\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ee0c6e4 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ee0c6e4\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ff85659\" data-id=\"ff85659\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f00ffc3 elementor-widget elementor-widget-text-editor\" data-id=\"f00ffc3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<p>Huntington\u2019s disease (HD) human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the disease study (molecular mechanisms, large-scale drug screening, and toxicological studies). iPSC lines ICGi007-A (<a href=\"https:\/\/hpscreg.eu\/cell-line\/ICGi007-A\">https:\/\/hpscreg.eu\/cell-line\/ICGi007-A<\/a>), ICGi018-A (<a href=\"https:\/\/hpscreg.eu\/cell-line\/ICGi018-A\">https:\/\/hpscreg.eu\/cell-line\/ICGi018-A<\/a>) and ICGi033-A (<a href=\"https:\/\/hpscreg.eu\/cell-line\/ICGi033-A\">https:\/\/hpscreg.eu\/cell-line\/ICGi033-A<\/a>) from HD patients with 47,38 and 77 CAG triplets respectively in the <em>HTT<\/em> gene were generated from blood mononuclear cells by non-integrating episomal vectors. The iPSC lines retained the mutation, expressed pluripotency markers, had a normal karyotype and displayed in vitro differentiation to the cell types of the three germ layers.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-f81a1da elementor-hidden-mobile elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"f81a1da\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-cd7d10a\" data-id=\"cd7d10a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2ae0706 elementor-widget elementor-widget-image\" data-id=\"2ae0706\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"Diseased iPSC lines - Huntington\u2019s disease (47 CAG)\" data-elementor-lightbox-description=\"Characterization of ICGi007-A iPSC cell line. \nA. Morphology of iPSC colony.\nB. Immunofluorescence staining for pluripotency markers NANOG, OCT3\/4, SOX2, TRA-1-60.\nC. Quantitative analysis expression of pluripotency markers (NANOG, OCT4, SOX2) by RT-qPCR.\nD. Karyotype (G-banding) analysis (46, XX).\nE. Immunofluorescence staining for differentiation markers: VIM and COLL IV (mesoderm); NF200 (ectoderm); CK18 and GATA6 (endoderm).\nF. DNA fragment analysis of PCR-product detected 17 and 47 CAG repeats in PBMCs and ICGi007-A. All scale bars - 100\u202f\u03bcm.\n\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6Ijc0MSIsInVybCI6Imh0dHBzOlwvXC9zaXRlcy5pY2diaW8ucnVcL3pha2lhbmxhYi1pcHNjY29sbGVjdGlvblwvd3AtY29udGVudFwvdXBsb2Fkc1wvc2l0ZXNcLzMxXC8yMDIwXC8wOVwvRGlzZWFzZWQtaVBTQy1saW5lcy1IdW50aW5ndG9uXHUyMDE5cy1kaXNlYXNlLnBuZyJ9\">\n\t\t\t\t\t\t\t<img width=\"1200\" height=\"1733\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease.png\" class=\"attachment-full size-full\" alt=\"Diseased iPSC lines - Huntington\u2019s disease (47 CAG)\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease-208x300.png 208w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease-709x1024.png 709w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease-768x1109.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease-1064x1536.png 1064w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-74f5b62\" data-id=\"74f5b62\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-db95a5c elementor-widget elementor-widget-image\" data-id=\"db95a5c\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"Diseased iPSC lines - Huntington\u2019s disease (38 CAG)\" data-elementor-lightbox-description=\"Characterization of ICGi018-A iPSC cell line.\nA. Morphology of iPSC colony.\nB. Alkaline phosphatase staining.\nC. Quantitative analysis of pluripotency marker expression (SOX2, NANOG, OCT4) by qPCR.\nD. Immunofluorescence staining for pluripotency markers SSEA-4, OCT4, SOX2 and TRA-1-60.\nE. In vitro differentiation. Immunofluorescence staining for differentiation markers: NF200 and TUBB3 (ectoderm); aSMA and COLL I (mesoderm); HNF3b and TBX3 (endoderm).\nF. Karyotype (G-banding) analysis (46, XX). G. DNA fragment analysis of PCR-products detected 18 and 38 CAG repeats in PBMCs and ICGi018-A. All scale bars are 100 \u00b5m.\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6Ijc0MiIsInVybCI6Imh0dHBzOlwvXC9zaXRlcy5pY2diaW8ucnVcL3pha2lhbmxhYi1pcHNjY29sbGVjdGlvblwvd3AtY29udGVudFwvdXBsb2Fkc1wvc2l0ZXNcLzMxXC8yMDIwXC8wOVwvRGlzZWFzZWQtaVBTQy1saW5lcy1IdW50aW5ndG9uXHUyMDE5cy1kaXNlYXNlMS5wbmcifQ%3D%3D\">\n\t\t\t\t\t\t\t<img width=\"1200\" height=\"1572\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1.png\" class=\"attachment-full size-full\" alt=\"Huntington\u2019s disease (38 CAG)\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1-229x300.png 229w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1-782x1024.png 782w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1-768x1006.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1-1173x1536.png 1173w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-317da1d\" data-id=\"317da1d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-391f080 elementor-widget elementor-widget-image\" data-id=\"391f080\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"http:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2.png\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"Diseased iPSC lines-Huntington\u2019s disease\" data-elementor-lightbox-description=\"Characterization of ICGi033-A iPSC cell line.\nA. Morphology of iPSC colony. B. Alkaline phosphatase staining.\nC. Immunofluorescence staining for pluripotency markers NANOG, OCT4, SOX2 and TRA-1-60.\nD. Quantitative analysis of pluripotency marker expression (OCT4, NANOG, SOX2) by qPCR.E. Karyotype (G-banding) analysis (46, XY).\nF. In vitro differentiation. Immunofluorescence staining for differentiation markers: MAP2 and TUBB3 (ectoderm); aSMA and NKX 2.5 (mesoderm); FOXA2 and AFP (endoderm).\nG. DNA fragment analysis of PCR-products detected 17 and 77 CAG repeats in PBMCs and ICGi033-A. All scale bars are 100 \u00b5m.\" e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6IjIzOTEiLCJ1cmwiOiJodHRwczpcL1wvc2l0ZXMuaWNnYmlvLnJ1XC96YWtpYW5sYWItaXBzY2NvbGxlY3Rpb25cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL3NpdGVzXC8zMVwvMjAyMFwvMDlcL0Rpc2Vhc2VkLWlQU0MtbGluZXMtSHVudGluZ3RvbnMtZGlzZWFzZTIucG5nIn0%3D\">\n\t\t\t\t\t\t\t<img width=\"1200\" height=\"1733\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2.png\" class=\"attachment-full size-full\" alt=\"Diseased iPSC lines - Huntington\u2019s disease\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2-768x1109.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2-1064x1536.png 1064w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\"><\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-412cef2 elementor-hidden-desktop elementor-hidden-tablet elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"412cef2\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-358abb4\" data-id=\"358abb4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8489354 elementor-widget elementor-widget-image\" data-id=\"8489354\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img width=\"1200\" height=\"1733\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease.png\" class=\"attachment-full size-full\" alt=\"Diseased iPSC lines - Huntington\u2019s disease (47 CAG)\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease-208x300.png 208w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease-709x1024.png 709w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease-768x1109.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease-1064x1536.png 1064w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Characterization of ICGi007-A iPSC cell line.  A. Morphology of iPSC colony. B. Immunofluorescence staining for pluripotency markers NANOG, OCT3\/4, SOX2, TRA-1-60. C. Quantitative analysis expression of pluripotency markers (NANOG, OCT4, SOX2) by RT-qPCR. D. Karyotype (G-banding) analysis (46, XX). E. Immunofluorescence staining for differentiation markers: VIM and COLL IV (mesoderm); NF200 (ectoderm); CK18 and GATA6 (endoderm). F. DNA fragment analysis of PCR-product detected 17 and 47 CAG repeats in PBMCs and ICGi007-A. All scale bars - 100\u202f\u03bcm.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-a13e705\" data-id=\"a13e705\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3bbbe65 elementor-widget elementor-widget-image\" data-id=\"3bbbe65\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img width=\"1200\" height=\"1572\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1.png\" class=\"attachment-full size-full\" alt=\"Huntington\u2019s disease (38 CAG)\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1-229x300.png 229w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1-782x1024.png 782w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1-768x1006.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntington\u2019s-disease1-1173x1536.png 1173w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Characterization of ICGi018-A iPSC cell line.A. Morphology of iPSC colony. B. Alkaline phosphatase staining. C. Quantitative analysis of pluripotency marker expression (SOX2, NANOG, OCT4) by qPCR. D. Immunofluorescence staining for pluripotency markers SSEA-4, OCT4, SOX2 and TRA-1-60. E. In vitro differentiation. Immunofluorescence staining for differentiation markers: NF200 and TUBB3 (ectoderm); aSMA and COLL I (mesoderm); HNF3b and TBX3 (endoderm). F. Karyotype (G-banding) analysis (46, XX). G. DNA fragment analysis of PCR-products detected 18 and 38 CAG repeats in PBMCs and ICGi018-A. All scale bars are 100 \u00b5m.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-f59bcf0\" data-id=\"f59bcf0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8993ca9 elementor-widget elementor-widget-image\" data-id=\"8993ca9\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img width=\"1200\" height=\"1733\" src=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2.png\" class=\"attachment-full size-full\" alt=\"Diseased iPSC lines - Huntington\u2019s disease\" loading=\"lazy\" srcset=\"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2.png 1200w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2-768x1109.png 768w, https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-content\/uploads\/sites\/31\/2020\/09\/Diseased-iPSC-lines-Huntingtons-disease2-1064x1536.png 1064w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Characterization of ICGi033-A iPSC cell line. A. Morphology of iPSC colony. B. Alkaline phosphatase staining. C. Immunofluorescence staining for pluripotency markers NANOG, OCT4, SOX2 and TRA-1-60. D. Quantitative analysis of pluripotency marker expression (OCT4, NANOG, SOX2) by qPCR.E. Karyotype (G-banding) analysis (46, XY). F. In vitro differentiation. Immunofluorescence staining for differentiation markers: MAP2 and TUBB3 (ectoderm); aSMA and NKX 2.5 (mesoderm); FOXA2 and AFP (endoderm). G. DNA fragment analysis of PCR-products detected 17 and 77 CAG repeats in PBMCs and ICGi033-A. All scale bars are 100 \u00b5m.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Huntington\u2019s disease (HD) human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the disease study (molecular mechanisms, large-scale drug screening, and toxicological studies). iPSC lines ICGi007-A (https:\/\/hpscreg.eu\/cell-line\/ICGi007-A), ICGi018-A (https:\/\/hpscreg.eu\/cell-line\/ICGi018-A) and ICGi033-A (https:\/\/hpscreg.eu\/cell-line\/ICGi033-A) from HD patients with 47,38 and 77 CAG triplets respectively in the HTT gene were generated from blood mononuclear [&hellip;]<\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":[],"categories":[2],"tags":[59,56],"_links":{"self":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/551"}],"collection":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/comments?post=551"}],"version-history":[{"count":59,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/551\/revisions"}],"predecessor-version":[{"id":2429,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/posts\/551\/revisions\/2429"}],"wp:attachment":[{"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/media?parent=551"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/categories?post=551"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.icgbio.ru\/zakianlab-ipsccollection\/wp-json\/wp\/v2\/tags?post=551"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}