1miRNAs ID | miRNA sequences | 0 | 1 | 2 | 3 | 2RPKM | 7Log2 Ratio | 8Species | |||||||||
miR-5p | miR-3p | Reads | Reads | Reads | Reads | 3C | 41d | 52d | 63d | 1d/C | 2d/C | 3d/C | |||||
Constitutive high expressed miRNAs | |||||||||||||||||
miR-162a | ucgauaaaccucugcauccag | 78 | 115 | 41 | 51 | 480,654 | 708,656 | 252,651 | 314,274 | 0.56 | -0.93 | -0.61 | Pt | ||||
miR-164a | uggagaagcagggcacgugca | 17 | 22 | 23 | 25 | 114,831 | 148,604 | 155,359 | 168,869 | 0.37 | 0.44 | 0.56 | Mt, Ptc, Bd | ||||
miR-164f | uggagaagcagggcacgugca | 1412 | 1381 | 1697 | 1554 | 7,085,152 | 6,929,600 | 8,515,229 | 7,797,682 | -0.03 | 0.27 | 0.14 | Mt, Ptc, Bd | ||||
miR-167h | ugaagcugccagcaugaucug | aggucaugcuguaguuucauc | 61 | 36 | 34 | 48 | 535,652 | 316,122 | 298,560 | 421,496 | -0.76 | -0.84 | -0.35 | At, Pp, Pt | |||
miR-169c | cagccaaggaugacuugccgg | 11 | 11 | 15 | 7 | 103,492 | 103,492 | 141,126 | 65,859 | 0.00 | 0.45 | -0.65 | At, Os, Mt, Ppe, Gm, Pto, Peu | ||||
miR-169g | uagccaaggaugacuugccua | 74 | 45 | 102 | 114 | 618,864 | 376,336 | 853,028 | 953,385 | -0.72 | 0.46 | 0.62 | At, Os, Mt, Ppe, Gm, Pto, Peu | ||||
miR-169i | uggugauaaggguguagcucug | ugagucgcucuuaucacucaug | 60 | 44 | 107 | 84 | 585,412 | 429,302 | 1,043,984 | 819,576 | -0.45 | 0.83 | 0.49 | At, Os, Mt, Ppe, Gm, Pto, Peu | |||
miR-394 | uuggcauucuguccaccucc | 6 | 4 | 7 | 0 | 57,477 | 38,318 | 67,056 | – | -0.58 | 0.22 | – | Pto, Ptc, Gm | ||||
miR-397b | uuauugagugcagcguugaug | 3 | 4 | 4 | 3 | 26,790 | 35,720 | 35,720 | 26,790 | 0.42 | 0.42 | 0.00 | At, Os, Ppe, Pto | ||||
miR-408 | cagggaugaggcagagcaugg | cugcacugccucuucccuggc | 392 | 427 | 608 | 470 | 1,939,279 | 2,112,429 | 3,007,861 | 2,325,156 | 0.12 | 0.63 | 0.26 | Os, At, Mt, Ppe, Pto, Ptc | |||
miR-414 | ucauccucaucaucaucgucc | 29 | 22 | 27 | 32 | 288,288 | 218,701 | 268,406 | 318,110 | -0.40 | -0.10 | 0.14 | – | ||||
miR-435 | uuauccgguauuggaguuga | 84 | 74 | 133 | 65 | 670,562 | 590,733 | 1,061,724 | 518,888 | -0.18 | 0.66 | -0.37 | – | ||||
miR-437 | aaaguuagagaaguuugacuu | 149 | 139 | 150 | 80 | 737,124 | 687,652 | 742,071 | 395,771 | -0.10 | 0.01 | -0.90 | – | ||||
miR-439d | ugucgaaccgcgguuguucga | 5 | 8 | 3 | 8 | 53,762 | 86,020 | 32,257 | 86,020 | 0.68 | -0.74 | 0.68 | – | ||||
miR-812a | gacggacgguuaaacguuggac | 517 | 517 | 507 | 318 | 2,328,137 | 2,328,137 | 2,283,105 | 1,432,007 | 0.00 | -0.03 | -0.70 | – | ||||
miR-812b | gacggacgguuaaacguuggac | 988 | 1060 | 1129 | 1503 | 6,124,093 | 6,570,384 | 6,998,078 | 9,316,308 | 0.10 | 0.19 | 0.61 | – | ||||
miR-812c | gacggacgguuaaacguuggac | 199 | 209 | 263 | 380 | 1,278,624 | 1,342,877 | 1,689,840 | 2,441,594 | 0.07 | 0.40 | 0.93 | – | ||||
miR-812d | gacggacgguuaaacguuggac | 335 | 334 | 322 | 220 | 2,307,210 | 2,300,323 | 2,217,677 | 1,515,183 | 0.00 | -0.06 | -0.61 | – | ||||
miR-812e | gacggacgguuaaacguuggac | 541 | 528 | 483 | 382 | 3,353,375 | 3,272,795 | 2,993,864 | 2,367,818 | -0.04 | -0.16 | -0.50 | – | ||||
miR-815b | aaggggauugaggagauuggg | 217 | 202 | 478 | 141 | 1,758,937 | 1,637,351 | 3,874,524 | 1,142,903 | -0.10 | 1.14 | -0.62 | |||||
miR-816 | gugacauauuuuacuacaac | 11 | 7 | 22 | 10 | 150,534 | 95,795 | 301,069 | 136,849 | -0.65 | 1.00 | -0.14 | |||||
miR-818c | aaucccuuauauuaugggacgg | 15 | 20 | 9 | 9 | 89,807 | 119,743 | 53,884 | 53,884 | 0.42 | -0.74 | -0.74 | – | ||||
miR-818e | aaucccuuauauuaugggacgg | 824 | 918 | 1275 | 956 | 3,859,033 | 4,299,262 | 5,971,198 | 4,477,227 | 0.16 | 0.63 | 0.21 | – | ||||
miR-820b | ucggccucguggauggaccag | 35 | 64 | 22 | 0 | 193,094 | 353,086 | 121,373 | – | 0.87 | -0.67 | – | |||||
miR-821a | aagucaucaacaaaaaaguugaau | 135 | 192 | 169 | 148 | 519,179 | 738,388 | 649,935 | 569,174 | 0.51 | 0.32 | 0.13 | – | ||||
miR-821b | aagucaucaacaaaaaaguugaau | 24 | 33 | 22 | 25 | 88,118 | 121,162 | 80,775 | 91,789 | 0.46 | -0.13 | 0.06 | – | ||||
Low expressed miRNAs | |||||||||||||||||
miR-156a | ugacagaagagagugagcac | 0 | 0 | 1 | 0 | – | – | 10,537 | – | – | – | – | At, Td, Tt, Hv, Os, Peu, Ppe, Pto | ||||
miR-156c | ugacagaagagagugagcac | gcucacuucucucucugucagc | 0 | 2 | 0 | 0 | – | 14,144 | – | – | – | – | – | At, Td, Tt, Hv, Os, Peu, Ppe, Pto | |||
miR-159c | auuggauugaagggagcucca | 0 | 1 | 5 | 0 | – | 5,349 | 26,745 | – | – | – | – | At, Peu, Os, Ppe | ||||
miR-160b | ugccuggcucccuguaugcca | gcgugcaaggagccaagcaug | 5 | 10 | 3 | 0 | 37,904 | 75,809 | 22,743 | – | 1.00 | -0.74 | – | Me, Me, Peu, Ppe, Pto, Ptc | |||
miR-160d | ugccuggcucccuguaugcca | gcgugcgaggagccaagcaug | 0 | 0 | 0 | 1 | – | – | – | 7,421 | – | – | – | Me, Me, Peu, Ppe, Pto, Ptc | |||
miR-164b | uggagaagcagggcacgugca | 0 | 7 | 1 | 0 | – | 67,671 | 9,667 | – | – | – | – | Mt, Pt, Bd | ||||
miR-164d | uggagaagcagggcacgugcu | 0 | 0 | 1 | 0 | – | – | 11,210 | – | – | – | – | Mt, Pt, Bd | ||||
miR-167i | ugaagcugccagcaugaucug | agaucauguugcagcuucacu | 0 | 0 | 0 | 2 | – | – | – | 10,485 | – | – | – | At, Ppe, Pto, Peu, Me, Me | |||
miR-169j | uagccaaggaugacuugccug | 0 | 1 | 0 | 0 | – | 8,430 | – | – | – | – | – | At, Os, Mt, Ppe, Gm, Pto, Peu | ||||
miR-169k | uagccaaggaugacuugccug | 1 | 0 | 0 | 0 | 6,755 | – | – | – | – | – | – | At, Os, Mt, Ppe, Gm, Pto, Peu | ||||
miR-171c | ggauauuggugcgguucaauc | ugauugagccgugccaauauc | 0 | 0 | 2 | 0 | – | – | 21,288 | – | – | – | – | At, Td, Os, Os, Mt, Ppe, Pto | |||
miR-319b | uuggacugaagggugcuccc | 0 | 2 | 0 | 0 | – | 10,698 | – | – | – | – | – | At, Os, Os, Pto | ||||
miR-395e | gugaaguguuugggggaacuc | 1 | 0 | 0 | 0 | 11,974 | – | – | – | – | – | – | Os, Ppe, Pto | ||||
miR-395h | gugaaguguuugggggaacuc | 0 | 0 | 1 | 0 | – | – | 9,408 | – | – | – | – | Os, Ppe, Pto | ||||
miR-396e | uccacaggcuuucuugaacug | augguucaagaaagcccauggaaa | 3 | 0 | 0 | 1 | 17,181 | – | – | 5,727 | – | – | -1.58 | At, Os, Mt, Ppe | |||
miR-397a | ucauugagugcagcguugaug | 0 | 0 | 0 | 2 | – | – | – | 18,487 | – | – | – | At, Os, Mt, Ppe, Pto, Peu | ||||
miR-397a | ucauugagugcagcguugaug | 0 | 0 | 0 | 2 | – | – | – | 18,487 | – | – | – | At, Os, Mt, Ppe, Pto, Peu | ||||
miR-399a | ugccaaaggagaauugcccug | 0 | 0 | 0 | 1 | – | – | – | 7,072 | – | – | – | Mt, Pto, Pto | ||||
miR-419 | ugaugaaugcugacgauguug | 0 | 14 | 4 | 0 | – | 143,227 | 40,922 | – | – | – | – | – | ||||
miR-426 | uuuuggaaguuuguccuuacg | 0 | 0 | 0 | 2 | – | – | – | 14,240 | – | – | – | |||||
miR-814b | cacuucauaguacaacgaaucu | 3 | 0 | 0 | 0 | 42,719 | – | – | – | – | – | – | – |
List of miRNAs that are constitutive pattern of high expression and low expression level. 1 Bold in miRNAs ID, rice specific drought responsive miRNAs; 2 RPKM, Reads Per Kilobase of transcript per Million mapped reads; 3 C, control; 4 1d, drought treatment for 1 day; 5 2d, drought treatment for 2 days; 6 3d, drought treatment for 3 days; 7 log2 ratio, log2(drought treatment / control), 8 Red, up-regulation by drought; Blue, down-regulation by drought Abbreviations: At, Arabidopsis thaliana; Bd, Brachypodium distachyon; Gm, Glycine max; Hv, Hordeum vulgare; Mt, Medicago truncatula; Me, Manihot esculenta; Pv, Phaseolus vulgaris; Peu, Populus euphratica; Ptc, Populus trichocarpa; Ppe, Prunus persica; Pte: Populus tremula; Pto, Populus tomentosa; Td, Triticum dicoccoides; Tt, Triticum turgidum; Os, Oryza sativa; Vu, Vigna unguiculata; Zm, Zea mays