Polymorphic microsatellite markers used in the QTL analysis performed on 6-month old F2 hybrid males (n=126) derived from a cross of ISIAH and WAG rats (total 149 markers). Position of markers is given in megabases (Mb) according to RGSC Genome Assembly v 5.0.
N |
Маrker |
Position on chromosome,Mb |
Primers, 5′->3′ |
An.temоС |
Expectedlengthof PCRfragment, n |
Distance between adjacent markers,Mb |
||
Chromosome 1 (length of chromosome 289.8 Mb) |
||||||||
1 |
D1Rat150 |
|
|
64 |
238 |
31.1 |
||
2 |
D1Rat234 |
48.6 |
GGGTACACTGGACTGGGAAAGCTGCCATTTAGTCTGGCTT |
62 |
152 |
10.7 |
||
3 |
D1Rat196 |
59.28 |
TGCTTTTCA
|
62 |
177 |
47.8 |
||
4 |
D1Rat30 |
|
|
64 |
223 |
31.0 |
||
5 |
D1Rat183 |
|
|
64 |
226 |
21.1 |
||
6 |
D1Rat131 |
|
|
64 |
176 |
22.7 |
||
7 |
D1Rat54 |
|
|
64 |
173 |
42.3 |
||
8 |
D1Rat168 |
224.22 |
AAGGAGCCACTAACTGTTCCC TCTCCAAAGCGGCTGAGTAT
|
64 |
210 |
16.4 |
||
9 |
D1Rat117 |
|
|
64 |
125 |
11.6 |
||
10 |
D1Rat76 |
|
|
64 |
144 |
20.0 |
||
11 |
D1Rat81 |
|
|
64 |
169 |
8.96 |
||
12 |
D1Rat86 |
281.20 |
AATACATGATGCTGTGGATTGG ACCCATTCCCACACCTGTAC
|
64 |
143 |
|||
Chromosome 2 (length of chromosome 285.1 Mb) |
||||||||
1 |
D2Rat3 |
|
|
62 |
127 |
39.4 |
||
2 |
D2Rat194 |
47.82 |
AGAGTGGATTTGAGAGCAGCTTTCTTTCTGTCTGGTTCTTCC |
60 |
220 |
19.2 |
||
3 |
D2Rat17 |
|
|
62 |
129 |
33.2 |
||
4 |
D2Rat75 |
|
|
58 |
121 |
25.6 |
||
5 |
D2Rat143 |
|
|
60 |
245 |
47.6 |
||
6 |
D2Rat224 |
|
|
62 |
160 |
41.9 |
||
7 |
D2Rat157 |
|
|
62 |
213 |
33.1 |
||
8 |
D2Rat88 |
|
|
58 |
128 |
19.8 |
||
9 |
D2Rat66 |
|
|
60 |
175 |
13.7 |
||
10 |
D2Rat70 |
|
|
58 |
165 |
|||
Chromosome 3 (length of chromosome 183.4 Mb) |
||||||||
1 |
D3Rat56 |
|
|
63 |
175 |
13.35 |
||
2 |
D3Rat194 |
15.95 |
GGTATCACTGCTCCACTCCCTGTGAGGACTGTTTGAATCT |
60 |
220 |
39.3 |
||
3 |
D3Rat130 |
55.23 |
AGAGAGTTGCCAAAATCATTCGTTATTGCCCATGGTTTGTAG
|
60 |
205 |
32.6 |
||
4 |
D3Rat31 |
|
|
60 |
173 |
11.5 |
||
5 |
D3Rat27 |
|
|
60 |
172 |
21.1 |
||
6 |
D3Rat111 |
120.44 |
ACTTTACAACCACCTCTTCAG
|
60 |
159 |
11.0 |
||
7 |
D3Rat71 |
131.46 |
GACGGGACGAGAAGAGATCAAGTTGTTTGTGGCATGCACAC |
62 |
130 |
23.7 |
||
8 |
D3Rat146 |
|
|
62 |
145 |
|||
Chromosome 4 (length of chromosome 248.3 Mb) |
||||||||
1 |
D4Rat136 |
|
|
62 |
241 |
36.9 |
||
2 |
D4Rat17 |
|
|
56 |
135 |
13.0 |
||
3 |
D4Rat20 |
59.52 |
CCTCAGAGGTCAGCATCTCCTGTGGTCTCTTCTTCCCTAG |
62 |
150 |
78.3 |
||
4 |
D4Rat27 |
|
|
60 |
125 |
13.2 |
||
5 |
D4Rat76 |
|
|
62 |
174 |
24.4 |
||
6 |
D4Rat177 |
|
|
61 |
201 |
21.3 |
||
7 |
D4Rat57 |
|
|
60 |
175 |
19.1 |
||
8 |
D4Rat61 |
|
|
62 |
157 |
18.2 |
||
9 |
D4Rat68 |
|
|
60 |
167 |
10.3 |
||
10 |
D4Rat140 |
|
|
58 |
146 |
|||
Chromosome 5 (length of chromosome 177.2 Mb) |
||||||||
1 |
D5Rat119 |
3.24 |
TGCCAATAGTTCCTCAGATAG
|
60 |
170 |
19.6 |
||
2 |
D5Rat188 |
22.82 |
TCTGACTTCTGGAAACTCTGAAGTTTCAGGAAGTCTGGTGC |
60 |
162 |
17.6 |
||
3 |
D5Rat70 |
40.45 |
CAGA
|
61 |
249 |
15.7 |
||
4 |
D5Rat6 |
|
|
60 |
159 |
12.6 |
||
5 |
D5Rat11 |
|
|
58 |
155 |
23.9 |
||
6 |
D5Rat85 |
|
|
58 |
247 |
17.2 |
||
7 |
D5Rat23 |
|
|
60 |
175 |
10.4 |
||
8 |
D5Rat115 |
|
|
62 |
203 |
20.6 |
||
9 |
D5Rat168 |
|
|
60 |
175 |
16.4 |
||
10 |
D5Rat93 |
|
|
60 |
247 |
14.3 |
||
11 |
D5Mco10 |
171.69 |
GGTACTAGAAATTAGCCGCTTTGTGAACCCCTGGAG
|
54 |
245 |
|||
Chromosome 6 (length of chromosome 156.9 Mb) |
||||||||
1 |
D6Rat80 |
|
|
60 |
244 |
29.9 |
||
2 |
D6Rat43 |
31.03 |
TTAGAAGGCTATTGCGTGCAGGGTCAGGCTCTTGACCATA |
60 |
172 |
17.0 |
||
3 |
D6Rat143 |
|
|
60 |
158 |
36.5 |
||
4 |
D6Rat23 |
|
|
58 |
141 |
18.9 |
||
5 |
D6Rat165 |
103.46 |
TTCAAAGGGGAGGGAAACTGTGTCCATCATGTGTCATTGTG
|
60 |
170 |
17.95 |
||
6 |
D6Rat66 |
|
|
62 |
202 |
15.2 |
||
7 |
D6Rat75 |
|
|
62 |
158 |
8.2 |
||
8 |
D6Rat101 |
144.76 |
CCAATACACTCCATGAATACC
|
60 |
150 |
|||
Chromosome 7 (length of chromosome 143.1 Mb) |
||||||||
1 |
D7Rat32 |
|
|
60 |
162 |
11.5 |
||
2 |
D7Rat150 |
36.59 |
CCAGATGGACTGCAAATTCACTCCCATCCCACTCAAA
|
60 |
170 |
17.6 |
||
3 |
D7Rat51 |
|
|
63 |
126 |
18.9 |
||
4 |
D7Rat165 |
|
|
62 |
217 |
19.4 |
||
5 |
D7Rat42 |
92.87 |
GCTGGAGCAGCAAGCTAAGAACCATGCCACTGAAAGAGGA |
62 |
190 |
22.1 |
||
6 |
D7Rat67 |
|
|
65 |
203 |
23.1 |
||
7 |
D7Rat173 |
|
|
62 |
208 |
|||
Chromosome 8 (length of chromosome 132.4 Mb) |
||||||||
1 |
D8Rat58 |
|
|
64 |
174 |
18.4 |
||
2 |
D8Rat53 |
21.87 |
CGCACAGGTAGGTATGCACATCCCAGGATGCTAATGAAGG
|
62 |
156 |
36.4 |
||
3 |
D8Rat149 |
|
|
62 |
139 |
2.8 |
||
4 |
D8Rat99 |
61.1 |
TGCAGAGAAAACAAGGGAGCACCACGAAGGTAAGCAATGG |
60 |
196 |
9.9 |
||
5 |
D8Rat93 |
|
|
58 |
126 |
18.1 |
||
6 |
D8Rat21 |
|
|
63 |
186 |
11.3 |
||
7 |
D8Rat19 |
|
|
60 |
149 |
14.3 |
||
8 |
D8Rat76 |
|
|
62 |
223 |
|||
Chromosome 9 length of chromosome 121.4 Mb) |
||||||||
1 |
D9Rat44 |
|
|
62 |
175 |
12.6 |
||
2 |
D9Rat79 |
15.43 |
CTCAGCAGGTAGGGCTTTTGACCCAGATAAATGCCAGGTG |
62 |
215 |
12.5 |
||
3 |
D9Rat70 |
|
|
58 |
190 |
19.0 |
||
4 |
D9Rat63 |
|
|
62 |
237 |
25.0 |
||
5 |
D9Rat15 |
|
|
60 |
137 |
5.85 |
||
6 |
D9Rat55 |
77.74 |
CATAAGACCATTTGTGTATGCATCAATAAAATCCTAACAGAAAG
|
58 |
138 |
15.4 |
||
7 |
D9Rat5 |
93.17 |
TGCATTTCTTGTCTGTCTCCACCTCTACACATGTGCCATGG
|
60 |
108 |
8.0 |
||
8 |
D9Rat100 |
|
|
58 |
91 |
16.7 |
||
9 |
D9Rat47 |
|
|
62 |
170 |
|||
Chromosome 10 (length of chromosome 110.6 Mb) |
||||||||
1 |
D10Rat67 |
|
|
60 |
202 |
12.6 |
||
2 |
D10Rat47 |
15.99 |
TTTTCCCCTTCTTTC
|
60 |
165 |
6.3 |
||
3 |
D10Rat43 |
|
|
60 |
149 |
47.7 |
||
4 |
D10Rat58 |
|
|
62 |
173 |
13.2 |
||
5 |
D10Wox16 |
83.20 |
ACCCCACAATCCAACACTATACGCAGGATCTAGTCTCAGCCC |
64 |
127 |
10.4 |
||
6 |
D10Rat17 |
|
|
60 |
172 |
2.1 |
||
7 |
D10Rat135 |
95.70 |
GCATGTCCTGGCACTTTTGT
|
65 |
155 |
|||
Chromosome 11 (length of chromosome 93.5 Mb) |
||||||||
1 |
D11Rat87 |
1.04 |
CT
|
60 |
154 |
14.2 |
||
2 |
D11Rat28 |
15.26 |
GATTTCCCTCCTCACTGCAAATATTC
|
60 |
249 |
18.8 |
||
3 |
D11Rat15 |
|
|
60 |
173 |
17.0 |
||
4 |
D11Rat7 |
|
|
62 |
160 |
15.2 |
||
5 |
D11Rat38 |
|
|
58 |
171 |
23.8 |
||
6 |
D11Rat50 |
|
|
62 |
236 |
|||
Chromosome 12 (length of chromosome 54.4 Mb) |
||||||||
1 |
D12Rat2 |
|
|
63 |
175 |
6.1 |
||
2 |
D12Rat91 |
19.95 |
CACAC
|
62 |
160 |
14.25 |
||
3 |
D12Rat93 |
|
|
60 |
132 |
20.1 |
||
4 |
D12Rat44 |
|
|
63 |
203 |
|||
Chromosome 13 (length of chromosome 118.7 Mb) |
||||||||
1 |
D13Rat120 |
|
|
60 |
141 |
57.2 |
||
2 |
D13Rat34 |
98.97 |
AGCACACGCCAAGTTTTTCC
|
60 |
220 |
|||
Chromosome 14 (length of chromosome 115.1 Mb) |
||||||||
1 |
D14Rat5 |
|
|
62 |
190 |
5.55 |
||
2 |
D14Rat78 |
17.42 |
ATGTCCTCCTCCGACCTTCCCAACCTGGAATGCAGAATG
|
60 |
142 |
10.1 |
||
3 |
D14Rat55 |
27.51 |
TTTGATGGGTTTGTTTACTTGGGGTAGAGTGATATAGGGAGAC
|
63 |
219 |
38.0 |
||
4 |
D14Rat88 |
|
|
62 |
173 |
20.5 |
||
5 |
D14Rat18 |
|
|
63 |
167 |
|||
Chromosome 15 (length of chromosome 114.5 Mb) |
||||||||
1 |
D15Rat76 |
|
|
60 |
133 |
17.3 |
||
2 |
D15Rat80 |
30.31 |
ATTGTCTGTGGGGCATTCTGAGGGGTTATT
|
60 |
175 |
24.6 |
||
3 |
D15Rat112 |
|
|
58 |
143 |
12.2 |
||
4 |
D15Rat68 |
|
|
62 |
132 |
20.0 |
||
5 |
D15Rat23 |
87.16 |
GTGGCTAACCTTGAATGTTCT
|
62 |
137 |
25.9 |
||
6 |
D15Rat29 |
113.1 |
CATGACCTATTTGCATTTGGC
|
60 |
153 |
|||
Chromosome 16 (length of chromosome 90.0 Mb) |
||||||||
1 |
В16Rat87 |
4.08 |
GACAGGTACTTAGACAGTCGTCCTCCTGCTTCTTGATTGG
|
60 |
160 |
6.2 |
||
2 |
D16Rat32 |
10.28 |
GAGAATCTAAGTGCTGCCGCTCCGTTAGGTGCTTTGTTCA |
60 |
125 |
13.4 |
||
3 |
D16Rat73 |
|
|
60 |
125 |
8.9 |
||
4 |
D16Rat99 |
|
|
60 |
205 |
17.3 |
||
5 |
D16Rat96 |
|
|
60 |
201 |
18.8 |
||
6 |
D16Rat58 |
|
|
60 |
171 |
11.0 |
||
7 |
D16Rat48 |
|
|
60 |
150 |
|||
Chromosome 17 (length of chromosome 91.8 Mb) |
||||||||
1 |
D17Rat107 |
11.84 |
TGTCATGTGACCAGGTGTGG
|
62 |
180 |
17.8 |
||
2 |
D17Rat12 |
29.61 |
GATTGGAGCCTTCATTAGAAAGGGCAA
|
62 |
158 |
15.9 |
||
3 |
D17Mgh3 |
45.53 |
TCAATGTAAGTTCAAAACTCACGTAAAGCACCTGTCTAGTATG |
60 |
130 |
5.6 |
||
4 |
D17Mgh5 |
51.17 |
TGGAGTGTGGACTGAAAATTGGAACAAATTGCAGGGCTATGG |
62 |
145 |
14.6 |
||
5 |
D17Mit4 |
65.76 |
GGATGAGTAGACAGGAGTGGCTTTGTTCTGTTTGGTATGCTC |
62 |
174 |
22.15 |
||
6 |
D17Wox8 |
87.91 |
GGGCTCAGTTCAAGTGAGAGCAGGTGACTCCCTTAATCGC
|
62 |
111 |
|||
Chromosome 18 (length of chromosome 86.3 Mb) |
||||||||
1 |
D18Rat111 |
|
|
60 |
249 |
9.7 |
||
2 |
D18Rat106 |
13.19 |
TGTGCACCCAAATATTTTTAGTTTTCAGGCAACCGTTCA
|
60 |
114 |
19.1 |
||
3 |
D18Rat22 |
32.31 |
CCCTGATCCATTGCCTCTGC
|
65 |
150 |
8.2 |
||
4 |
D18Rat96 |
|
|
62 |
247 |
7.20 |
||
5 |
D18Mgh1 |
47.73 |
GTGATTAGAACATATGCAAAAGGCAATCTCCTTGACTCTGAAATGACC
|
66 |
126 |
13.0 |
||
6 |
D18Rat40 |
|
|
62 |
152 |
25.4 |
||
7 |
D18Rat1 |
86.11 |
GGATCAGTCAGGAAGTCGGAAAGGATGTGTTTCACCCACC
|
61 |
115 |
|||
Chromosome 19 (length of chromosome 72.7 Mb) |
||||||||
1 |
D19Rat28 |
|
|
60 |
198 |
19.3 |
||
2 |
D19Rat47 |
24.43 |
GGCAAGCTCCACAGAATAAAACAGAGGAAAGGCAAGATAG
|
58 |
228 |
7.3 |
||
3 |
D19Rat26 |
31.72 |
AAGGGGA
|
62 |
160 |
19.3 |
||
4 |
D19Rat91 |
51.02 |
TGAAGAAGAGAACCAACTCCCATCCATACTACTCTTCATTC
|
58 |
238 |
12.0 |
||
5 |
D19Rat6 |
|
|
62 |
154 |
|||
Chromosome 20 (length of chromosome 57.7 Mb) |
||||||||
1 |
D20Rat47 |
|
|
64 |
139 |
19.9 |
||
2 |
D20Rat30 |
2
|
|
61 |
135 |
25.2 |
||
3 |
D20Rat16 |
|
|
60 |
155 |
|||
Chromosome X (length of chromosome 154.5 Mb) |
||||||||
1 |
DXRat44 |
|
|
60 |
223 |
12.8 |
||
2 |
DXRat85 |
25.9 |
GTGGGTCTTTGACTGACTTCGGTAGAGAGCCATAGAGAAC |
60 |
224 |
19.2 |
||
3 |
DXRat26 |
|
|
62 |
152 |
43.5 |
||
4 |
DXRat93 |
|
|
58 |
200 |
12.9 |
||
5 |
DXRat34 |
|
|
62 |
106 |
27.4 |
||
6 |
DXRat140 |
|
|
58 |
155 |
12.35 |
||
7 |
DXRat104 |
141.25 |
GACAGCATAGTTCCACAATAC
|
60 |
156 |
10.75 |
||
8 |
D0Mco53 |
152.0 |
GAGGTGGTTTACTGATCTACCAACCTCTGGAACAGTT
|
62 |
210 |