Олигонуклеотиды для ПЦР-РВ

Oligo used for RT PCR

Oligo name

Sequence

Oligo length

b.

Temp of annealing

Product length ,

b.

PpiaL

5’-ACCGTGTTCTTCGACATCAC-3’

20

60

130

PpiaR

5’-GAACCCTTATAGCCAAATCCT-3’

21

60

EgfrL

5’-CGGAACTTACAGGAAATTCTG-3’

21

60

117

EgfrR

5’-CATGTTGCTCAGAAAGACATC-3’

21

60

ComtL

5’-GACAA↓AGTCACCATCCTCAAT-3’

21

60

122.

ComtR

5’- GAAGTGTGTCTGGAAGGTAG -3’

20

60

EgfL

5’- ACATCAAGCACGGCACGATT-3’

20

60

112

EgfR

5’-ATTCTTCCCAGTGTGTTTGTC-3’

21

60

Scnn1aL

5’-CTCCTTCTGTCACGATGGTC-3’

20

62

141

Scnn1aR

5’-GTAGCAGCATGAGAAGTGTG-3’

20

60

bENaCL

5’-CTGGGACTGAATTTGGTCTCA-3’

21

62

127

bENaCR

5’-TCTCTAATGAAGGGGTATGTC-3’

21

60

Ephx2L

5’-TATGTGACAGTGAAGCCAGG-3’

20

60

192

Ephx2R

5’-GAAGATGAGTCTCCATAGCC-3’

20

60

MlrL

5’-CCAAGGTACTTCCAGGATTTA-3’

21

60

159

MlrR

5’- TTGGCTGTTCGTGTGTTTGTA -3’

21

60

 aENaCr1

5’-AGTGTAATTGTTCTGCAAGGAC-3’

22

62

aENaCwt

5’-GCTCCTGGGGCTATTGCTAT-3’

20

62

238

aENaCa

5’-GCTCCTGGGGGCGCCTTCTC-3’

20

70

215

aENaCb

5’-GCTCCTGGGTGTGATCAACT-3’

20

62

159

– division of exon

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