Oligo used for RT PCR
|
Oligo name |
Sequence |
Oligo length b. |
Temp of annealing |
Product length , b. |
|
PpiaL |
5’-ACCGTGTTCTTCGACATCAC-3’ |
20 |
60 |
130 |
|
PpiaR |
5’-GAACCCTTATAGCCAAATCCT-3’ |
21 |
60 |
|
|
EgfrL |
5’-CGGAACTTACAGGA↓AATTCTG-3’ |
21 |
60 |
117 |
|
EgfrR |
5’-CATGTTGCTCAGAAAGACATC-3’ |
21 |
60 |
|
|
ComtL |
5’-GACAA↓AGTCACCATCCTCAAT-3’ |
21 |
60 |
122. |
|
ComtR |
5’- GAAGTGTGTCTGGAAGGTAG -3’ |
20 |
60 |
|
|
EgfL |
5’- ACATCAAGCACG↓GCACGATT-3’ |
20 |
60 |
112 |
|
EgfR |
5’-ATTCTTCCCAGTGTGTTTGTC-3’ |
21 |
60 |
|
|
Scnn1aL |
5’-CTCCTTCT↓GTCACGATGGTC-3’ |
20 |
62 |
141 |
|
Scnn1aR |
5’-GTAGCAGCATGAGAAGTGTG-3’ |
20 |
60 |
|
|
bENaCL |
5’-CTGGGACTGAATTTG↓GTCTCA-3’ |
21 |
62 |
127 |
|
bENaCR |
5’-TCTCTAATGAAGGGGTATGTC-3’ |
21 |
60 |
|
|
Ephx2L |
5’-TATGTGACAGTGAAG↓CCAGG-3’ |
20 |
60 |
192 |
|
Ephx2R |
5’-GAAGATGAGTCTCCATAGCC-3’ |
20 |
60 |
|
|
MlrL |
5’-CCAAGGTACTTCCAG↓GATTTA-3’ |
21 |
60 |
159 |
|
MlrR |
5’- TTGGCTGTTCGTGTGTTTGTA -3’ |
21 |
60 |
|
|
aENaCr1 |
5’-AGTGTAATTGTTCTGCAAGGAC-3’ |
22 |
62 |
– |
|
aENaCwt |
5’-GCTCCTGGG↓GCTATTGCTAT-3’ |
20 |
62 |
238 |
|
aENaCa |
5’-GCTCCTGGG↓GGCGCCTTCTC-3’ |
20 |
70 |
215 |
|
aENaCb |
5’-GCTCCTGGG↓TGTGATCAACT-3’ |
20 |
62 |
159 |
↓ – division of exon