Biosensors

pMito-Grx1-roGFP2-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the mitochondrial sensor of glutathione redox potential Grx1-roGFP2

pCyto-Grx1-roGFP2-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the cytosolic sensor of glutathione redox potential Grx1-roGFP2

pCyto-roGFP2-Orp1-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the cytosolic sensor of hydrogen peroxide roGFP2-Orp1

pMito-Grx1-roGFP2-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the mitochondrial sensor of glutathione redox potential Grx1-roGFP2

pCyto-Grx1-roGFP2-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the cytosolic sensor of glutathione redox potential Grx1-roGFP2

pCyto-roGFP2-Orp1-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the cytosolic sensor of hydrogen peroxide roGFP2-Orp1

pMito-roGFP2-Orp1-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the mitochondrial sensor of hydrogen peroxide roGFP2-Orp1

pXBP1-TagRFP-ERSS-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the XBP1-TagRFP sensor of endoplasmic reticulum stress

pXBP1-luc2-hRluc-ERSS-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the XBP1-luc2-hRluc sensor of endoplasmic reticulum stress

pMito-roGFP2-Orp1-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the mitochondrial sensor of hydrogen peroxide roGFP2-Orp1

pXBP1-TagRFP-ERSS-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the XBP1-TagRFP sensor of endoplasmic reticulum stress

pXBP1-luc2-hRluc-ERSS-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the XBP1-luc2-hRluc sensor of endoplasmic reticulum stress

p1chr-SHL-EGFP-ATF6-donor

Donor plasmid designed for homologous recombination at the human 1 chromosome safe harbor locus. Doxycycline-dependent expression of the EGFP-ATF6 sensor of endoplasmic reticulum stress

pAAVS1-TRE-CMV-GCaMP6f

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the calcium sensor GCaMP6f

pAAVS1-TRE-CMV-GCaMP6m

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the calcium sensor GCaMP6m

p1chr-SHL-EGFP-ATF6-donor

Donor plasmid designed for homologous recombination at the human 1 chromosome safe harbor locus. Doxycycline-dependent expression of the EGFP-ATF6 sensor of endoplasmic reticulum stress

pAAVS1-TRE-CMV-GCaMP6f

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the calcium sensor GCaMP6f

pAAVS1-TRE-CMV-GCaMP6m

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the calcium sensor GCaMP6m

pAAVS1-TRE-CMV-GCaMP6s

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the calcium sensor GCaMP6s

pCasper3-BG-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the Caspase-3 apoptosis sensor Casper3-BG

pCasper3-GR-donor

Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the Caspase-3 apoptosis sensor Casper3-GR