iPSC-derived hepatocyte-like cells are a convenient tool for hepatotoxic screening of potential medical products, basic research of liver diseases pathogenesis, and for the development of cell technologies for the liver diseases treatment. We used a monolayer differentiation protocol to obtain hepatocyte-like cells from human iPSCs using specific growth factors (Tasnim et al., 2015, doi: 10.1016/j.biomaterials.2015.08.002). During 6 days of the differentiation iPSCs were converted into a definitive endoderm by the activation of Nodal and Wnt signaling pathways. At this stage, specific factors (GATA4, HNF4A, HNF3B / FOXA2, TBX3) were expressed. At the next stage, there was a transition to hepatoblasts for one week (SOX17, AFP, ALBUMIN). At the terminal stages of differentiation hepatocytes expressed AAT, CYP1A1, TRANSFERRIN.
