Isogenic lines constructions – Zakian Lab iPSC collection

Donor plasmid for homologous recombination in the HTT locus for Huntington’s disease modeling. The plasmid contains long homology arms to the first exon of HTT, which flank 215 CAG repeats. We have a set of identical donor plasmids differing from each other by the number of CAG triplets.

Donor plasmid for homologous recombination in the HTT locus for Huntington’s disease modeling. The plasmid contains long homology arms to the first exon of HTT, which flank 215 CAG repeats. We have a set of identical donor plasmids differing from each other by the number of CAG triplets.