Parkinson’s disease

Diseased iPSC lines - Parkinson’s disease

Parkinson’s disease (PD) is one of the most common neurodegenerative diseases. The development of the disease is associated with the death of neurons, mainly in the substantia nigra, which produce the neurotransmitter dopamine. Insufficiency of dopamine causes a range of severe symptoms, among them: bradykinesia, muscle rigidity and tremor, which leads to disability of patients. Using non-integrating episomal vectors the iPSC lines ICGi015-A (https://hpscreg.eu/cell-line/ICGi015-A) and ICGi015-B (https://hpscreg.eu/cell-line/ICGi015-B) were generated from blood mononuclear cells of PD patient, carrying three SNPs, associated with PD development. These iPSC lines can be used to study the cellular and molecular aspects of PD pathogenesis and to test new drugs.

Diseased iPSC lines - Parkinson’s disease
Characterization of ICGi015-A and ICGi015-B iPSC lines. A. Morphology of the iPSC colonies. B. Immunofluorescence staining for pluripotency markers NANOG, OCT3/4, SOX2, and TRA-1-60. C. Analysis of pluripotency markers (OCT4, SOX2, NANOG) expression by RT-qPCR. D. Karyotypes (G-banding) (46, XX) of ICGi015-A and ICGi015-B iPSC lines. E. In vitro differentiation. Immunofluorescence staining for differentiation markersαSMA (mesoderm), NF200 (ectoderm), and AFP (endoderm) after spontaneous differentiation of ICGi015-A and ICGi015-B iPSC lines in embryoid bodies. The scale bar A, B and E - 100μm.

Parkinson’s disease (PD) is one of the most common neurodegenerative diseases. The development of the disease is associated with the death of neurons, mainly in the substantia nigra, which produce the neurotransmitter dopamine. Insufficiency of dopamine causes a range of severe symptoms, among them: bradykinesia, muscle rigidity and tremor, which leads to disability of patients. Using non-integrating episomal vectors the iPSC lines ICGi015-A (https://hpscreg.eu/cell-line/ICGi015-A) and ICGi015-B (https://hpscreg.eu/cell-line/ICGi015-B) were generated from blood mononuclear cells of PD patient, carrying three SNPs, associated with PD development. These iPSC lines can be used to study the cellular and molecular aspects of PD pathogenesis and to test new drugs.