The study of neurodegenerative diseases such as Parkinson’s disease has long been hampered by the lack of models that accurately reproduce human brain cells in vitro. iPSC-differentiated dopaminergic neurons carry the same genetic signature as the donor and allow researchers to interrogate the causes of Parkinson’s disease. We use several protocols to differentiate human iPSCs into midbrain-patterned neural progenitor cells (NPCs), astrocytes, and dopaminergic neurons (de Rus Jacquet, 2019; Niclis, J.C., 2016; Samata, B., 2015).