iPSC-derived endothelial cells serve as a convenient model to study molecular mechanisms of vascular diseases, provide an opportunity for screening of novel drugs, creating of vascularized 3D-organoids and vascular regeneration. We generate endothelial cells from human PSCs using a monolayer differentiation approach (Gu, 2018, doi: 10.1002/cphg.64 with changes). The first step is efficiently mesoderm generation via small‐molecule activation of WNT signaling for 4 days. A subsequent endothelial cells specification is carried out for another 4 days through specific growth factors. After 8 days of differentiation, mature endothelial cells are enriched by magnetic‐activated cell sorting for the surface marker CD31. The obtained endothelial cells express of specific markers: CD 31, vWF, VEGFR2, ANG1, bFGF, CXCR4, SDF1, form of tube‐like structures and uptake of acetylate low‐density lipoprotein.