iPSC-derived cardiomyocytes are a convenient tool for studying molecular mechanisms of inherited cardiovascular diseases. To generate cardiomyocytes from patient-specific iPSCs, we use a protocol based on the WNT signaling pathway activation (through GSK3β inhibition with CHIR99021) followed by WNT repression with IWP2 or Wnt-C59 (Burridge et al., Nat. Methods, 2014). The cells obtained in the course of the directed cardiac differentiation contracted spontaneously and expressed main cardiomyocyte markers such as sarcomeric α-actinin, cardiac troponin T, β-myosin heavy chain, and NKX2.5 transcription factor.
