Hypertrophic cardiomyopathy (HCM) is one of the most common cardiovascular diseases and is characterized by abnormal thickness of left ventricular wall and interventricular septum, diastolic dysfunction, progressive heart failure, and a high risk of arrhythmias…
Control iPSC line – iMA-1LThe induced pluripotent stem cell (iPSC) line iMA-1L was generated from human embryonic dermal fibroblasts using episomal vectors expressing pluripotency factors. The episomes were delivered into the cells by nucleofection (NHDF…
Isogenic HD modelHuntington’s disease (HD) is a severe neurodegenerative disorder caused by a CAG triplet expansion in the first exon of the HTT gene. Here we generated isogenic iPSC-based HD model by introducing 69 CAG…
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by death of motor neurons. To date, neither etiology nor pathogenesis of ALS is clear, which leads to the absence of an effective treatment strategy. Animal…
Hypoxia-inducible factors (HIFs) are transcription factors that play an important role in the adaptive response to hypoxic conditions. HIFs are stabilized in hypoxia but degraded in normoxia. HIF-2α subunit is involved in the regulation of…
Patient-specific induced pluripotent stem cells (iPSCs) capable of differentiation into required cell type are a promising model for studying various pathological processes and development of new therapeutic approaches. However, no conventional strategies for using iPSCs…
The study of molecular mechanisms of hereditary multigene diseases is a complex, largely unsolved problem. This is due to a large variety of genes, a large number and variety of mutation that are directly related…
Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the mitochondrial sensor of glutathione redox potential Grx1-roGFP2
Donor plasmid designed for homologous recombination at the AAVS1 locus. Doxycycline-dependent expression of the human alpha-synuclein protein with 3×FLAG and 2×Strep-Tag II tags on C terminus